珍稀濒危植物银鹊树胚性细胞悬浮系的建立和植株再生

Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Tapiscia sinensis Oliv

以银鹊树未成熟种子为试材,对银鹊树胚性愈伤组织诱导和胚性细胞悬浮培养的最佳培养条件进行研究,初步建立了银鹊树胚性细胞悬浮系与植株再生体系。将银鹊树未成熟种子子叶胚接种在添加1.0 mg/L 2,4-D、0.5 g/L活性炭的MS基本培养基上,诱导胚性愈伤组织。将诱导得到的胚性愈伤组织置于添加0.2 mg/L 6-BA、0.05 mg/L NAA和3 g/L蔗糖的MS液体培养基上振荡培养,由此建立了增殖速度快、分散程度好、稳定性较强的胚性细胞悬浮体系。将悬浮培养获得的子叶胚转到不含任何植物生长调节物质的MS固体培养基中,可以长成正常植株。

We preliminary established embryonic cells suspension system and plant regeneration system by exploring the best conditions of embryogenic callus abduction and embryogenic cells cultivation,with Tapiscia sinensis Oliv immature seed tree as an experimental material.Embryogenic callus could be originated from immature seeds on MS solid medium augmented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D) and 0.5 g/L active carbon.Obtained callus were subsequently cultured in MS liquid medium with 0.2 mg/L 6-benzylaminopurine(6-BA),0.05 mg/L naphthalene acetic acid(NAA) and 3 g/L sucrose for establishing rapid-growing and well-dispersed embryogenic cell suspension system.The cotyledon embryos from suspension culture could grow into normal plants in liquid MS medium without any plant growth regulating substances.