转大豆Na^+/H^+逆向转运蛋白GmNHX1基因植株的获得

Obtaining Transgenic Soybean Plants with Na^+/H^+ Antiporter(Gm NHX1)

制约大豆生产的因素很多,土壤盐渍化就是其中之一。大量研究证明过表达NHX逆向转运蛋白可以提高植物的耐盐性。为获得耐盐性良好的转基因大豆材料,我们将大豆Na^+/H^+逆向转运蛋白(GmNHX1)基因构建到植物表达载体pCAMBIA3300上,应用农杆菌介导法将GmNHX1导入大豆品种黑农56和黑农59中,共获得18个转基因株系,并对T_1代转基因株系进行了PCR和实时荧光定量PCR检测。PCR结果表明转基因后代株系呈阳性的植株有4株;经Real-time PCR检测该4个PCR阳性转基因株系的G NHX1基因表达水平均高于对照株系;200 mmol/L NaCl溶液的盐试结果表明;对照植株的生长速度滞缓于4个转基因株系的生长速度。

There are many factors restricting the soybean yields and soil salinization is one of them. Lots of studies have shown that NHX can improve the salt tolerance of plants. In order to obtain transgenic soybean with good salt tolerance, we inserted the Na^+/H^+Antiporter GmNHX1 gene from Soybean into dicotyledon expression vector p CAMBIA3300, and then transferred it into soybean cultivars Heinong 56 and Heinong 59 by Agrobacterium-mediated method. 18 transgenic plantlets were obtained. PCR and real-time fluorescent quantitative PCR were used to detect the T1 generation. PCR analysis showed that 4 plants were confirmed as transgenic plants; Real-Time PCR analysis showed that the expression level of GmNHX1 in these four lines was higher than untransformed control lines. The result of 200 mmol/L salt solution detection showed that the growth rate of untransformed control lines was slower than the four transgenic lines.