4种杜鹃幼苗高温胁迫下蛋白表达差异

Differences in the Protein Expression of Four Rhododendron Species Seedlings under High Temperature Stress

为进一步明确杜鹃幼苗的耐热机制,为耐热杜鹃育种提供分子依据,本研究比较了映山红、满山红、马银花和云锦杜鹃4种耐热性不同的杜鹃幼苗在高温胁迫下蛋白质组分变化的差异。研究发现,高温胁迫导致4种杜鹃叶片蛋白质表达出现了明显差异,主要表现为蛋白质表达的下调或消失、蛋白质表达的上调或特异蛋白的出现及波动表达。其中耐热性最强的映山红有60个蛋白位点出现差异表达,其中包括30个特异表达蛋白位点和8个表达上调的蛋白位点,其次是马银花,有55个蛋白位点出现差异表达,10个蛋白质表达上调,27个特异蛋白。高温胁迫下映山红和马银花表达上调和特异表达的蛋白位点也明显高于另外2种热敏感杜鹃。

In order to make clear the mechanism of the response of Rhododendron seedlings to high temperature and provide the molecular basis for breeding of heat-resistant Rhododendron, the changes in the protein expression of four Rhododendron species with varied heat sensitivity were studied. The results indicated that high temperature resulted in the difference of the protein expression, which inhibited down-regulated expression, disappearance of some proteins, up-regulated expression, the induction of stress proteins and fluctuation expression of some proteins.A quantitative analysis, using PDQuest software, revealed that a total 60 protein spots were differentially expressed on heat-stress-treated R. simsii leaves: 30 protein spots were either newly induced or detected and 8 were up-regulated. There were 55 differentially expressed proteins: 27 protein spots were either newly induced or detected and 10 were up-regulated. High temperature induced more differently expressed proteins compared with heat-sensitivity Rhododendron.