基于SRAP标记的油菜菌核病抗性资源遗传多样性分析

Analysis on Genetic Diversity of Rapeseed Germplasms(Brassica campestris)with Resistance to Sclerotinia Disease Based on SRAP Markers

为提高油菜菌核病抗性育种效率和合理利用资源,本研究运用本实验室筛选的40对多态性高、条带清晰的SRAP引物,分析汉中市农科所经过茎秆接种鉴定的菌核病抗性表现较好的43份油菜材料的遗传多样性。采用NTSYS-pc2.10e软件中的DICE法计算遗传相似系数,UPGMA法构建聚类图。结果显示,SRAP标记共检测到335个多态性条带,平均每个标记检测到8个多态性条带,每个SRAP位点的遗传多态性信息含量(PIC)介于0.31~0.99之间,平均值为0.69。供试43份油菜材料遗传相似系数主要分布在0.65~0.77之间,遗传相似度较高,表明供试油菜材料间遗传差异较小,应进一步加强抗性资源筛选,拓宽现有油菜遗传背景,有助于抗菌核病油菜品种选育。

In order to improve the efficiency of breeding work of rapeseed with resistant to Sclerotinia sclerotiorum and make ration al use of resources, forty pairs of high polymorphic, clear bands SRAP primers selected by our laboratory were used to analysis the genetic diversity of Sclerotinia sclerotiorum resistant rapeseed identified with stem inoculation method by Hanzhong Institute of Agricultural Sciences. Genetic similarity coefficient and clustering dendrogram were calculated and constructed by using Dice and UPGMA(unweighted pair-group method with arithmetic average) methods in NTSYS-pc2.10 e software. The results showed that, in total,335 polymorphism bands were detected by SRAP markers, the average numbers of polymorphism bands per primer was 8, the PIC value of each SRAP loci were between 0.31~0.99 with the average value of 0.69. Genetic similarity coefficient of the 43 rapeseed samples distributed mainly between 0.65 to 0.77. The genetic similarity of the materials was relatively high, the genetic variation was small. Thus, it's of great importance to enhance the resistant material screening and enrich the genetic base of current rapeseed in order to be helpful for the breeding of Sclerotinia sclerotiorum resistant rapeseed.