脑红蛋白在感染性休克早期大鼠脑组织中的动态变化

Protein content of neuroglobin in rat brain with septic shock

目的探讨感染性休克早期大鼠脑组织中脑红蛋白（neuroglobin,NGB）含量的动态变化在感染性休克中可能发挥的作用。方法健康雄性SD大鼠60只,12~15周龄,体重300~350g,随机分为两组,每组30只。M组经尾静脉注射脂多糖（LPS）10mg/kg制备大鼠感染性休克模型,当SBP下降幅度达到基础值的40%时认为感染性休克模型复制成功;C组给予等量生理盐水。分别在给LPS前（T_0）、给LPS后1h（T_1）、2h（T_2）、4h（T_3）、6h（T_4）采用ELISA法检测血清中TNF-α、IL-6浓度和NGB含量,Western blot法检测脑组织中NGB、半胱天冬酶-3（caspase-3）的含量。结果与C组比较,T1~T4时M组血清中的TNF-α、IL-6浓度明显增高（P〈0.05）,脑组织中caspase-3含量明显增加（P〈0.05）;T_3、T_4时血清和脑组织中NGB含量明显增高（P〈0.05）。结论感染性休克后大鼠脑组织发生了炎症损伤、细胞凋亡病理学改变,脑红蛋白在感染性休克所致的损伤脑组织中含量增加,推测其可能发挥了内源性神经保护功能。

Objective To observe the expression of neuroglobin in brain of rat with septic shock,and study the protective effect of neuroglobin.Methods Sixty male SD rats were randomly divided into two groups,30 rats in each.The rats in group M were injected LPS 10mg/kg through tail vein to establish septic shock model,the success standard of septic shock was systolic blood pressure dropping to 60% of the basic values;the control group were given equal volume of NS.The levels of rat TNF-α,IL-6 and NGB in serum at the time points of before LPS injection（T_0）,1h（T_1）,2h（T_2）,4h（T_3）and 6h（T_4）after LPS injection were measured using ELISA kits.NGB and caspase-3protein expression in brain were determined using Western blotting.Results Compared with group C and T_0,the TNF-α,IL-6concentrations in serum and caspase-3in brain were increased significantly at T_1-T_4（P〈0.05）,NGB protein content in serum and brain at T_3 and T_4 were increased significantly（P〈0.05）.Conclusion Cell apoptosis and inflammatory response are discovered in the brain during septic shock.NGB is over-expressed in brain of rats with septic shock,which may play a role of protective function.