树兰茎段丛生芽快繁体系的建立

Establishment of rapid propagation system for clustered buds of Epidendrum stem

【目的】建立茎段诱导丛生芽途径的树兰快速繁殖技术体系,为树兰种苗生产及工厂化育苗奠定基础。【方法】以树兰当年生分蘖苗的带芽茎段为外植体,以MS为基本培养基,研究不同质量浓度6-BA、NAA和IBA组合对树兰茎段丛生芽诱导、增殖及生根的影响。【结果】不同质量浓度6-BA和NAA组合对树兰茎段丛生芽的诱导与增殖均有显著影响,树兰茎段丛生芽诱导最适宜培养基为MS+1.0mg/L 6-BA+0.5mg/L NAA,诱导率最高达到73.13%;增殖最适宜培养基为MS+3.0mg/L 6-BA+1.0mg/L NAA,增殖系数最高达到5.2。不同质量浓度NAA和IBA组合对树兰组培苗生根有显著影响,生根最适宜培养基为MS+1.0mg/L NAA+1.5mg/L IBA,生根率可达100%,平均根数7.78条/株,株高7.44cm,茎粗2.82mm。【结论】建立了树兰茎段诱导丛生芽的组织培养快繁体系,在该体系下丛生芽的诱导率和增殖率均较高,生根状况良好。

【Objective】A simple and efficient micro-propagation system was established for direct clustered buds induction fromEpidendrumstems to improve seedling production and industrialization.【Method】The stem section with axillary bud of current-year tillering Epidendrum was used as explant and MS medium was used as basal culture medium to investigate the effects of 6-BA,NAA and IBA at different concentrations on induction,multiplication,and rooting of clustered buds.【Result】Combinations of 6-BA and NAA at different concentrations had significantly influence on induction and proliferation of clustered buds of Epidendrumstems.The optimal medium for clustered buds induction was MS＋1.0mg/L 6-BA＋0.5 mg/L NAA with the highest induction rate of 73.13%.The optimal medium for proliferation was MS＋3.0mg/L 6-BA＋1.0 mg/L NAA with the highest proliferation coefficient of 5.2.NAA and IBA combinations also had significantly influence on rooting of Epidendrum.The optimal medium for shootrooting was MS＋1.0 mg/L NAA＋1.5 mg/L IBA with the rooting rate of 100%,the average rooting number of 7.78,the shoot height of 7.44 cm,and the stem diameter of 2.82 mm.【Conclusion】A rapid propagation system for clustered buds of Epidendrumstem was established,which provided high induction rate and proliferation coefficient of clustered buds and good shoot rooting.