两种不同的检测方法对80例乙型肝炎病毒血清标志物的结果分析

Analysis on the results of two different detection methods in detecting 80 cases of HBV serum markers

目的分析两种不同检测方法对80例乙型肝炎病毒血清标志物的检测结果。方法选择2013年8月至2015年12月在我院进行诊治的80例乙型肝炎患者作为研究对象,所有患者均抽取空腹静脉血后分离血清标本,采用RT-PCR法检测HBV-DNA情况,采用ELISA法检测HBeAg阳性情况。结果 RT-PCR判定为大三阳45例,小三阳25例,其他10例;RT-PCR判定为HBV-DNA阳性67例,占比83.75%;ELISA法判断阳性70例,占比87.50%,不同方法判断的阳性率对比差异无统计学意义(P>0.05)。结论 RT-PCR与ELISA法检测乙型肝炎病毒血清标志物都有较好的检测效果,可以对乙型肝炎病毒的传染状况进行检测,有很好的应用价值。

Objective To analyze the results of two different detection methods in detecting 80 cases of HBV serum markers. Methods Eighty cases of patients with hepatitis B in our hospital from August 2013 to December 2015 were selected as study objects. The fasting venous blood of all the patients were collected, and their serum samples were separated. Then, RT-PCR test was used to detect the HBV-DNA, while the ELISA method was used to detect the positive rate of HBeAg. Results There were 45 cases with ＂great three positive＂, 25 cases with ＂small three positive＂ and 10 cases with other HBV carrier detected by RT-PCR method. The HBV-DNA positive patients were 67 cases detected by RT-PCR test, accounting for 83.75%, while the positive patients were 70 cases detected by ELISA method, accounting for 87.50%, there was no significant difference in the positive rates of different methods （P〉0.05）. Conclusion Both RT-PCR and ELISA method have a good effectiveness on detecting HBV serum markers. They can detect the status of hepatitis B virus infection effectively, and have a very good application value.