2Gyγ射线照射对大鼠外周血淋巴细胞差异基因表达的影响

The differential gene expression in rat lymphocytes after exposure to 2 Gy γ-rays

目的 探讨γ射线照射对大鼠外周血淋巴细胞基因表达的影响，筛选与辐射损伤密切相关的差异表达基因及生物学通路。方法 采用基因芯片技术对2 Gy 60Co γ射线离体和整体照射后6 h大鼠外周血淋巴细胞差异表达基因进行筛选，利用基因本体（gene ontology，GO）和京都基因与基因组百科全书（KEGG）数据库对差异表达基因进行生物信息学分析，并应用实时荧光定量PCR技术对基因芯片结果进行验证。结果 离体照射组与整体照射组筛选出差异表达3倍以上的共同差异基因55条；共同差异表达基因涉及的生物学通路有6个；2条差异表达基因的PCR扩增结果与芯片结果具有良好的一致性。结论 γ射线离体和整体照射可引起大鼠外周血淋巴细胞基因差异表达，涉及到多种生物学过程，表明淋巴细胞对辐射损伤的应答反应是复杂的、多基因协同作用的结果。

Objective To investigate the impacts of γ-ray radiation on gene expressions of peripheral blood lymphocytes in SD rats, and to screen differential expression genes and biological pathways closely related to radiation injury. Methods The differential gene expression of peripheral blood lymphocytes in SD rats was selected by microarray at 6 h after 2 Gy 60Co γ-ray exposure in vitro and in vivo. Bioinformatics analysis of the differentially expressed genes was performed by using Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） databases. Real-time quantitative PCR was applied to verify the screen results of the microarray. Results Fifty-five genes with three times over-expressed level were screened out from the radiation groups both in vitro and in vivo and they were involved in 6 signaling pathways. There were two differentially expressed genes of microarray assay were verified by PCR assay. Conclusions Differential expressed genes in the peripheral blood lymphocytes of SD rats could be induced by γ-ray radiation and they cooperatively contributed to a variety of biological processes.