蒙药复方述达格-4化学成分的分离与结构鉴定

Separation and identification of chemical constituents of Mongolian medicine compound Shudage-4

目的研究蒙药复方述达格-4的化学成分,阐明其发挥药效作用的物质基础。方法采用乙醇提取,硅胶、Sephadex LH-20柱、反相硅胶等各种柱色谱法及结晶法进行分离纯化,采用紫外光谱（UV）、红外光谱（IR）、质谱（MS）、核磁共振谱（NMR）等光谱波谱技术对分离得到的化合物进行结构鉴定。结果从蒙药复方述达格-4中分离鉴定了10个化合物,分别为1-苯基-7-（3-甲氧基-4-羟基）苯基-5-醇-3-庚酮（1）、豆甾醇（2）、山奈酚（3）、胡萝卜苷（4）、对甲氧基苯甲酸（5）、熊果酸（6）、齐墩果酸（7）、去氢木香烯内酯（8）、木香烯内酯（9）、去氢二异丁香油酚（10）。结论化合物1和化合物3~10均为首次从复方述达格-4分离得到,为阐明蒙药复方述达格-4的物质基础提供了重要的科学研究数据。

Objective To study the chemical constituents of Mongolian medicine compound Shudage-4,so as to find out material bases of its pharmacodynamic effects. Methods The chemical constitutes of Shudage-4 were extracted using ethanol extraction method,and were isolated and purified using technologies of silica gel,Sephadex LH-20 and reversed phase silica gel. Then the structures of the isolated compounds were identified by using different spectroscopic and chromatographic techniques including ultra violet spectroscopy（ UV）,infrared spectroscopy（ IR）,mass spectrometry（ MS） and nuclear magnetic resonance（ NMR）. Results There were 10 compounds isolated and identified from Mongolian medicine Shudage-4—1-phenyl-7-（ 3-methoxy-4-hydroxy） phenyl-5-ol-3-heptanone-one（ 1）,stigmasterol（ 2）,kaempferol（ 3）,daucosterol（ 4）,p-Anisic acid（ 5）,ursolic acid（ 6）,oleanic acid（ 7）,dehydrocostus lactone（ 8）,costunolide（ 9） and dehydrodiisoeugenol（ 10）. Conclusion The ten compounds,except stigmasterol,were first isolated from Shudage-4. This paper provided important scientific data for the material bases of Mongolian medicine Shudage-4.