顶空固相微萃取-气相色谱法测定尿中五氯酚

Determination of Pentachlorophenol in Human Urine by Headspace Solid Phase Microextraction Coupled with Gas Chromatography

[目的]建立顶空固相微萃取-气相色谱法测定尿中五氯酚的方法。[方法]利用顶空固相微萃取技术,富集尿中五氯酚,优化萃取条件和色谱柱,利用电子捕获检测器测定。[结果]该方法测定范围为0.50-5.00 mg/L,相关系数r=0.999 3,检出限为0.09 mg/L,平均回收率为97.6%-103.7%,精密度为1.63%-6.78%。[结论]顶空固相微萃取技术处理尿样简便快速,检出限低,结果准确可靠,可用于职业接触五氯酚人群尿中五氯酚的测定。

[Objective] To establish a method for pentachlorophenol determination in human urine by headspace solid phase microextraction coupled with gas chromatography. [Methods] Headspace solid phase microextraction technology was used to enrich pentachlorophenol in urine. The extraction conditions and the chromatographic column were optimized. Pentachlorophenol was determined with electron capture detector. [Results] The linear dection rang for pentachlorophenol in urine was 0.50-5.00 mg/L; the correlation coefficient was 0.999 3; and the limit of detection was 0.09 mg/L. The average recovery rate for pentachlorophenol was from 97.6% to 103.7%, with relative standard deviations of 1.63%-6.78%. [Conclusion] The method is featured by easy sample preparation, low limit of detection, and high accuracy, and suitable for the determination of pentachlorophenol in worker＇s urine.