谷胱甘肽S-转移酶P1基因多态性与支气管肺发育不良的关系研究

Relationship of Gene Polymorphisms of Glutathione S-Transferase P1 and Bronchopulmonary Dysplasia

目的探讨谷胱甘肽S-转移酶P1(GSTP1)基因多态性与支气管肺发育不良(BPD)的关系。方法选取2010年3月—2013年12月在武汉市第三医院新生儿科及华中科技大学同济医学院附属同济医院新生儿重症监护室(NICU)住院的患有BPD的早产儿60例为BPD组(其中男38例,女22例),及同期入住华中科技大学同济医学院附属同济医院NICU无肺部疾病的早产儿99例为对照组(其中男63例,女36例)。釆用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测GSTP1基因多态性。结果两组GSTP1基因型频率均符合Hardy-Weinbery平衡,具有群体代表性。两组GSTP1基因型频率和等位基因频率间差异均无统计学意义(P>0.05)。两组男性GSTP1基因型频率和等位基因频率间差异均无统计学意义(P>0.05)。结论 GSTP1基因多态性与BPD易感性无关。

Objective To investigate the relationship of gene polymorphisms of glutathione S- transferase P1（ GSTP1） and bronchopulmonary dysplasia. Methods 60 hospitalized premature infants with BPD in Neonatology Department in Wuhan Third Hospital, and Neonatal Intensive Care Unit（ NICU） of Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology from March 2010 to December 2013 were selected as BPD group（ 38 boys,22 girls）,and99 hospitalized premature infants without pulmonary disease in NICU of Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology at the same period were enrolled as control group（ 63 boys,36 girls）. The gene polymorphism of GSTP1 was detected by polymerase chain reaction- restriction fragment length polymorphism（ PCR-RFLP）.Results The genotype frequencies of GSTP1,with representativeness of the groups,were consistent with Hardy- Weinbery equilibrium. There was no significant difference in genotype frequency and allele frequency of GSTP1 between the two groups（ P〈0. 05）. There was no significant difference in genotype frequency and allele frequency of GSTP1 genotype between males in the two groups（ P〈0. 05）. Conclusion The gene polymorphism of GSTP1 is not associated with the susceptibility BPD.