尿液HCMV-DNA和血清HCMV-IgM检测对婴儿巨细胞病毒感染的诊断价值

The diagnostic value of urine HCMN-DNA and serum HCMV-IgM detection to infants with cytomegalovirus infection

目的：探讨尿液人巨细胞病毒‐DNA和血清人巨细胞病毒免疫球蛋白检测在婴儿巨细胞病毒感染中的诊断价值。方法对泉州儿童医院收治的856例巨细胞病毒感染疑似住院患儿，采用化学发光免疫法检测血清人巨细胞病毒免疫球蛋白，采用荧光定量聚合酶链反应法检测尿液中巨细胞病毒载量，并进行统计分析。结果尿人巨细胞病毒‐DNA 和血清人巨细胞病毒免疫球蛋白阳性检出率分别为30．72％和34．93％（ P＞0．05）；不同性别疑似感染率差异无统计学意义（P＞0．05），不同年龄组人巨细胞病毒免疫球蛋白与人巨细胞病毒‐DNA检测差异有统计学意义（P＜0．01）；不同年龄组荧光定量聚合酶链反应检测差异有统计学意义（P＜0．01），三个年龄组中＜28 d组阳性率最低，6个月‐1岁组最高。结论尿液人巨细胞病毒‐DNA和血清人巨细胞病毒免疫球蛋白联合检测，有助于人巨细胞病毒感染的诊断。

Objective To investigate the diagnostic value of urine HCMN‐DNA and serum HCMV‐IgM de‐tection to infants with cytomegalovirus infection .Methods Serum HCMV‐IgMs were detected with che‐moluminescence immunifaction and urine cytomegalovirus loads with FQ‐PCR in 856 infants with suspected cytomegalovirus infection hospitalized in Quanzhou Children＇s Hospital ,statistical analyses carried out . Results The positive detection rates of urine HCMN‐DNA and serum HCMV‐IgM were respectively 30 . 72% and 34 .93% （P〉0 .05）;suspected infection rate had no gender difference （P〉0 .05） ,there was age differences in urine HCMN‐DNA and serum HCMV‐IgM detection （P〈0 .05）;there was age differences in the FQ‐PCR （P〈 0 .01） ,positive rate in infants 〈 28 days was lowest and that in ones aged 6‐12 months highest .Conclusion Combined detection of urine HCM N‐DNA and serum HCM V‐IgM is con‐duce to the diagnosis of human cytomegalovirus infections .