苦荞中黄曲霉毒素B_1含量的3种测定方法的比较

COMPARISON OF 3 METHODS FOR THE DETERMINATION OF AFLATOXIN B_1 IN TARTARY BUCKWHEAT

以苦荞粉为原料，采用荧光光度法、HPLC和ELISA测定苦荞粉中黄曲霉毒素B，的含量，从而进行方法学比较。结果表明，3种方法的检测范围分别是1～25g／kg、0．2～200g／kg和0．1～5g／kg；平均相对误差分别是3．975％、4．288％和0．078％；平均加标回收率分别是79．91％、84．58％和90．11％；变异系数分别是7．803％、7．441％和0．358％；最低检测限分别是1．0g／kg、0．2g／kg和0．1g／kg。因此，荧光光度法的回收率、精密度最低，检测范围较ELISA的检测范围宽；HPLC标准曲线的线性关系最好，检测范围最宽；ELISA的准确度、回收率、精密度、灵敏度最高，但ELISA的检测范围最窄。

Aflatoxin is a kind of toxic metabolites of fungi, which is highly toxic and can damage the liver tissues of human and animals. Three methods for Aflatoxin B1 determination were compared in the present study including Fluorophotometry, HPLC and ELISA method, while buckwheat flour was used as raw material. The results showed that the detection range of fluorophotometry, HPLC and ELISA were 5-20 μg/kg, 0.2 -200 μg/kg and 0.1 -5 μg/kg, respectively; the average relative errors were 3.975%, 4.288% and 0.078%; the average standard recovery rates were 79.91%, 84.58% and 90.11% ;the variation coefficients were 7.803%, 7.441% and 0.358%; the lowest detection limits were 1.0 μg/kg, 0.2 μg/kg and 0.1 μg/kg. It was concluded that fluorophotometry had the lowest average recovery rate and precision, but the detection range was wider than ELISA;HPLC had the best curve linear relationship and the widest linearity range;ELISA had the best average recovery rate, precision and sensitivity, but the narrowest detection range.