市售肉苁蓉饮片的鉴别及苯乙醇苷类的含量测定

Determination of commercially available Cistanche pieces identification and phenylethanoid glycosides content

[目的]建立市售肉苁蓉饮片的高效液相色谱法(HPLC)指纹图谱,以期鉴别肉苁蓉真伪。建立HPLC方法测定其苯乙醇苷类含量。[方法]采用HPLC法,以标准药材肉苁蓉为参照图谱,对肉苁蓉饮片进行指纹图谱分析。采用Kromasil C18色谱柱,甲醇∶水(0.1%甲酸)梯度洗脱,检测波长330 nm。[结果]该方法精密度、稳定性、重现性较好,不同市场采购的肉苁蓉指纹图谱差异较大。苯乙醇苷类含量测定方法符合要求。[结论]福建、江苏和山东省购买的肉苁蓉饮片鉴定为伪品,其他省市购买的肉苁蓉饮片苯乙醇苷类含量符合要求,初步评价质量符合要求。

[Objective] Establish commercial Cistanche Pieces of HPLC fingerprint identification cistanche authenticity. Establish HPLC method for the determination of benzene in commercial cistanche glycosides content. [Methods] Standard Cistanehe herbs as a reference fingerprint, using high performance liquid chromatography to analyze the fingerprint of Cistanche Pieces; Using Kromasil Cls column,methanol： water （containing 0.1% formic acid） for gradient elution and wave length of detection was at 330 nm. [Results] The method precision, stability, good reproducibility, different market purchases of Cistanche fingerprints large differences. Phenylethanoid glycosides determination method meet the requirements. [Conclusion] Cistanche Pieces that purchase in Fujian, Jiangsu and Shandong province identified as counterfeit. Cistanche Pieces of the other provinces glycosides benzene content to purchase to meet the requirements, a preliminary evaluation of the quality to meet the requirements.