无糖基化修饰GLP-1-Fc(N126A)在酵母中的表达、纯化

Expression and Purification of Non-glycosylated Glp-1-Fc(N126A)Fusion Protein in Yeast Expression System

胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)是一种肠促胰岛素激素,具有很好的综合降糖效果,但其在血浆中的半衰期只有短暂几分钟,限制了GLP-1在临床方面的应用.为延长GLP-1的半衰期,合成了GLP-1-Fc融合基因,将N-糖基化作用的受体天冬酰胺突变为丙氨酸,构建了无糖基化修饰的GLP-1-Fc(N126A)融合表达载体,利用酵母表达系统成功表达了GLP-1-Fc(N126A)融合蛋白,并利用r Protein A获得了高纯度融合蛋白.

Glucagon-like peptide-1（GLP-1） is a kind of ducdenin, which has good and comprehensive hypoglycemic effect. The GLP-1 was easy to be degraded in plasma. To prolong the half-life of GLP-1, the GLP-1-Fc（N126A） was constructed through mutant N-glycosylation asparagine receptor into alanine. The GLP-1-Fc（N126A） fusion protein was obtained by yest expression system, and was purified by rPro-teinA.