摘要
目的探讨诱导痰Runt相关基因(Runx)3基因CpG岛甲基化在肺腺癌病情及预后评估中的价值。方法选择肺腺癌患者(LAC组)和非肿瘤患者(CON组)为研究对象,采用甲基化特异性聚合酶链反应(MSP)检测LAC组和CON组患者诱导痰Runx3基因CpG岛甲基化并比较其差异,比较LAC组肺腺癌患者不同临床病理特征中Runx3基因CpG岛甲基化的差异。比较LAC组患者诱导痰Runx3基因CpG岛甲基化与未甲基化患者R0切除率、无病生存期和3年生存率的差异。结果LAC组肺腺癌患者诱导痰Runx3基因CpG岛甲基化率显著高于CON组非肿瘤患者(62.5%比2.5%,P〈0.05)。LAC组肺腺癌患者在胸腔积液、分化程度、肿瘤最大径、淋巴结转移、远处转移和cTNM分期中的差异有统计学意义(χ2分别为5.861、4.417、5.273、4.110、10.240、10.559,均P〈0.05)。LAC组肺腺癌患者中Runx3基因CpG岛甲基化者R0切除率(72.0%比93.3%)、无病生存期[(1.7±0.4)年比(2.4±0.6)年]和3年生存率(40.0%比66.7%)均显著低于Runx3基因CpG岛未甲基化患者(χ2=4.083、t=6.261;χ=4.320,P〈0.05)。结论肺腺癌患者诱导痰Runx3基因CpG岛甲基化与病情及预后相关。
Objective To study the value of runt related transcription factor 3 (Runx3) gene CpG island methylation in induced sputum in condition and prognosis evaluation for lung adenocarcinoma. Meth- ods Methylation specific polymerease chain reaction (MSP) was used to detect the Runx3 gene promoter methylation by induced sputum in patient with lung adenocarcinoma ( LAC group) and non - cancer pa- tients (CON group). The Runx3 gene CpG island methylation rate in different clinicopathologieal factors was compared. The R0 radical resection, disease - free survival time and 3 - year survival rate between pa- tient with Runx3 gene CpG island methylation and unmethylation were compared. Results The Runx3 gene promoter methylation rate in LAC group was significantly higher than in CON group (62. 5% vs. 2. 5% , P 〈 0.05 ). The Runx3 gene promoter methylation rate in pleural effusion, degree of differentiation, tumor diameter, lymph node metastasis, distant metastasis and cTNM staging were significantly different (χ2 = 5.861, 4.417, 5.273, 4. 110, 10.240, 10.559, all P〈0.05). The R0 radical resection (72.0% vs. 93.3 % ), disease - free survival time [ ( 1.7 ± 0. 4) years vs. ( 2.4 ± 0. 6) years ] and 3 - year survival rate (40. 0% vs. 66. 7% ) in patient with Runx3 gene CpG island methylation were significantly lower than in patients with Runx3 gene CpG island unmethylation (χ2 =4. 083 ,t =6. 261 ,χ2 =4. 320, all P 〈0.05). Conclusion Runx3 gene CpG island methylation in induced sputum correlates with lung adenocareinoma' s condition and prognosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第12期2786-2788,共3页
Chinese Journal of Experimental Surgery
关键词
肺腺癌
Runt相关基因3
甲基化
Lung adenocarcinoma
Runt related transcription factor 3
Methylation