体外释放酶免疫技术检测结核分枝杆菌的临床应用分析

Clinical Application of Vitro Enzyme Immunoassay for Detection of Mycobacterium

目的探讨体外释放酶免疫技术在检测结核分枝杆菌中的临床应用价值。方法选择临床病例100例(肺内结核组80例,肺外结核组20例),采用体外释放酶免疫技术(TB-IGRA)、结核抗体试验(TB-AB)和痰涂片镜检检测,比较检测的阳性率以及灵敏度和特异度。结果 TB-IGRA检测肺内结核组、肺外结核组的阳性率高于TB-AB和痰涂片镜检检测,差异具有统计学意义(P<0.05),TB-IGRA、TB-AB、痰涂片镜检的灵敏度为92.3%、42.5%、29.8%,差异具有统计学意义(P<0.05);特异度差异不具有统计学意义(P>0.05)。结论TB-IGRA检测有更好的优越性和临床应用效果。

Objective To explore the clinical application value of vitro enzyme immunoassay for detection of tuberculosis mycobacterium. Methods TB- IGRA, TB-AB and sputum smear micrnscopy were performed in 100 cases （ 80 cases of pulmonary tuberculosis group and 20 cases ofextrapuhnonary tuberculosis group ）, the positive rate and the sensitivity and specificity of the test were compared. Results The positive rate of TB-IGRA in tuberculosis group and extranuclear tuberculosis group was significantly higher than that in TB-AB and sputum smear （P 〈 0.05 ）, TB-IGRA, TI3- AB and sputum the sensitivity of smear microscopy was 92.3%,, 42.5%, 29.8%, the difference was statistically siguificant （P 〈 0.05 ）, the specificity was not statistically significant （ P 〉 0.05 ） . Conclusion TB-IGP,.A detection has better superiority and clinical application.