分子生物学技术在细菌性阴道病病原学研究中的应用进展

Progress in Application of Molecular Biology Technology in the Study of Etiology in Bacterial Vaginosis

细菌性阴道病(BV)是育龄女性最常见的下生殖道感染性疾病,是妊娠并发症的危险因素,增加性传播疾病的危险性。在BV的病原学研究中发现,BV是由于患者阴道微生态失衡,阴道内占优势的乳酸杆菌数量减少,阴道防御力下降,而使其他多种病原微生物大量繁殖所致。近年来,以聚合酶链反应、16S rRNA序列分析为基础的分子生物学技术在细菌群落研究中得到了发展。该文主要介绍了BV病原学研究中所使用的几种分子生物学技术,以期为今后BV的研究提供更可靠的依据。

Bacterial vaginosis（BV） is the most common lower genital tract infective disease in reproductive age women,which is the risk factor for pregnancy complications and increases the risk of sexually transmitted diseases. The bacterial vaginosis etiology study found that BV occurrence is due to the vaginal microecological imbalance, the decrease in the number of vaginal predominant lactobacillus, decreased vaginal defense force of the patients, allowinga variety of pathogenic microorganisms to multiply. In recent years,based on the polymerase chain reaction（PCR） and 16S rRNA sequence analysis, molecular biology techniques have been developed in the bacterial study. With the purpose of providing more reliable basis in the bacterial vaginal research in the future, here is mainly to introduce several kinds of molecular biological techniques used in the study of pathogenic bacterial vaginosis.