肝纤维化大鼠肝气郁结证与肝藏血关系的研究

Study the relationship between liver qi stagnation and liver stores blood in liver fibrosis rats

目的:探讨中医藏象理论"肝藏血主疏泄"相关的肝脏生理病理生物学基础。方法:以四氯化碳皮下注射法建立肝纤维化肝气郁结证大鼠模型,采用肝脏超声检测和功能核磁灌注方法,检测肝气郁结证大鼠动态活体肝纤维化过程中脑部和肝脏血管血流速度、血管直径、血流量变化,同时进行大鼠大脑功能核磁分析。结果:在肝纤维化肝气郁结证病程中,大鼠肝动脉、肝静脉血管管径及血流量明显增加,海马及下丘脑脑区灌流量明显增加,且脑杏仁核异常激活。结论:脑部和肝脏血流变化与肝藏血理论之间具有相关性,肝藏血功能异常与肝脏和脑部血流动力学改变密切相关。

Objective： To explore the biological mechanism of ＂liver storing blood and Liver Controlling Disper＂. Methods：The rat liver fibrosis model was established by subcutaneous injection of carbon tetrachloride;The functional magnetic resonance imaging and liver uhra- sound were used to detect brain and liver blood flow velocity, the vein diameter and blood flow in the model rats. Results：The test result shows that vein diameter and blood flow of the hepatic artery and hepatic vein, the blood flow of hippocampus and hypothalamus were in- creased in liver fibrosis. Also abnormal activation of brain amygdala was found in brain functional magnetic analysis. Conclusion ： The results were proved to relate to ＂liver storing blood ＂theory closely. The abnormal function of ＂liver storing blood＂ was closely related to the hemody- namics changes of liver and brain.