红缘天牛对枣树6种挥发物EAG和嗅觉行为反应

EAG and Olfactory Behavioral Responses of Asias halodendri to Six Volatiles from Chinese Jujube Tree

为探明枣树挥发物吸引红缘天牛成虫的原因,应用触角电位仪和嗅觉仪分别测定红缘天牛成虫对6种枣树挥发物的电生理反应和嗅觉行为反应。结果表明:试验所选的6种化合物均能引起红缘天牛的触角电位反应,随着浓度增加,电生理反应逐渐增加,当刺激物的浓度达到100μg/μL时,红缘天牛对6种化合物的EAG达到最高值。红缘天牛雌雄成虫对正十一烷的触角电位反应相对值均为最大,分别可以达到2.34、2.29。在嗅觉行为反应测定中,正十一烷和乙酸叶醇酯对红缘天牛雌雄成虫产生明显的引诱作用。本研究为进一步研发红缘天牛植物源引诱剂提供了理论依据。

The aim is to verify the reasons of Zizyphus jujube（Chinese jujube tree） volatiles attracting Asias halodendri. The electroanten nogram and olfactory behavioral responses of A. halodendri to six volatiles fromChinese jujube tree were tested with the electroantennogram（EAG） and Y-tube olfactometer,respectively. Theresults showed that the six compound chemicals emanating from Z. jujube could induce obvious EAG responsesof male and female beetles. A. halodendri had the highest EAG relative value to the six compound chemicalswhen the concentration of stimulation got to 100 μg/μL. The EAG responses value of male and female A.halodendri to n-hendecane was the highest,being 2.34 and 2.29,respectively. As for olfactory behavioralresponse tests,the male and female beetles were attracted by n-hendecane and cis-3-hexenyl acetate. The research could provide a theoretical basis for developing botanical attractants to A. halodendri.