UPLC-MS/MS法测定鸡蛋、鸡肉中氯霉素药物残留

UPLC/MS/MS Method for Determination of Chloramphenicol Drug Residues in Eggs and Chicken

建立鸡肉和鸡蛋中氯霉素残留检测的UPLC-MS/MS方法。样品采用乙酸乙酯两部提取之后氮吹的方法;HESI源负离子模式扫描,在选择反应监测模式(SRM)下进行特征母-子离子对信号采集;根据保留时间和母离子及两个特征子离子信息进行定性分析,6 min内完成氯霉素的定量分析。结果显示,方法测定底限为0.1μg·kg-1,在1,3,5μg·kg-1添加水平下,鸡蛋加标回收率在80%~110%之间,鸡肉加标回收率在70%~100%之间,且两种样本批内变异系数小于10%。该方法前处理简便快速,分析速度快,灵敏度高。

The paper established the UPLC-MS/MS method to determine the residues of the chloramghenicol in the chickens and eggs. The samples were extracted by ethyl acetate for two times and blow dry through termovap sample concentrator. The samples were scanned by heated electro spray ionization （HESI）, and the signal collection of characteristic maternal ions and sub-ion pairs was conducted by the selective reaction monitor （SRM）. The qualitative analysis was conducted on the basis of retaining time, maternal ions and the two characteristic sub-ion, and the quantitative analysis of chloramghenicol was conducted in 6 minutes. The results showed that on the condition that the detection limit was 0.1μg·kg^-1 and 1, 3, 5 μg·kg^-1 addition level, the recovery rate in eggs and chickens is 80%-110% and 70%-100% respectively, and the coefficient of variation between the two kings of samples was less than 10%. The method has the advantage in simple and fast on the pretreatment, quick analysis and high sensitivity.