高效液相色谱法同时测定伤科胶囊中3种皂苷的含量

Simultaneous Determination of the Contents of Three Kinds of Saponins in ShangKe Capsules by High Performance Liquid Chromatography

目的：建立高效液相色谱法同时测定伤科胶囊中3种皂苷的含量。方法：采用高效液相色谱法，色谱柱为ODS柱（4．6mm×250μm，5μm，流动相A为乙腈，B为0．05％磷酸溶液，梯度洗脱，条件为：0-18min（20～35：80-65），18—23min（35—20：65-80），25min（20：80）；流速为1．0mL／min，检测波长203nm。结果：三七皂苷R1进样量在0．7758～24．8256μg（r=0．9997）、人参皂苷Rg1进样量在0．8520—27．2640μg（r=0．9998）、人参皂苷Rb1进样量在0．6432～20．5824μg（r=为0．9999）范围内线性关系良好，方法重复性及回收率均符合要求。结论：本法操作简便快捷、结果准确可靠，重复性好，适用于伤科胶囊中3种皂苷含量的测定。

Objective： To establish a determination method for the contents of three kinds of saponins in ShangKe capsules by high performance liquid chromatography. Methods： Methods of high performance liquid chromatography was used with ODS colurrm（4.6 mm×250 mm, 5 μm）. The mobile phase A was acetonitrile while B was 0.05% phosphoric acid solution. The gradient elution conditions were as follows： 0-18 min（20-35：80-65）, 18- 23min（35-20：65-80）, 25 rain （20：80）; At the same time, the flow rate was 1 mL/min, and the detection wavelength was 203 nm. Results： There was a good linear relationship of the sample size of notoginsenoside R1 in the range of 0.7758-24.825 6 g （r=0.999 7）, ginsenoside Rgl of 0.852 0-27.264 0 μg （r=0.999 8）, ginsenoside Rbj of 0.643 2- 20.582 4 μg（r=0.999 9）. Meanwhile, the repeatability and recovery of the method were in line with the requirements. Conclusion： This method is simple, rapid, accurate and reliable, and can be used for the detection of the content of three kinds ofsaponins in ShangKe capsules.