流动注射化学发光法检测蔬菜中乐果含量

Flow Injection Chemiluminescence Method for Detection of Dimethoate in Vegetables

在碱性介质下,过氧化氢氧化鲁米诺产生化学发光现象,乐果对该化学发光体系有较强的增敏作用,据此建立了流动注射化学发光测定乐果含量的快速分析方法。结果显示,乐果的质量浓度为0.01~10 mg/L时与发光强度成良好的线性关系。该方法的最低检出限为0.009 mg/L,对0.5 mg/L的乐果进行11次平行测定,相对标准偏差为2.1%。将该体系用于菜花样品的加标回收实验,回收率为86.7%~109.5%。

In alkaline medium, hydrogen peroxide oxidizes luminol and then produces chemilumines-cence. Dimethoate could enhance the chemiluminescence intensity strongly, and thus a quick and direct flow injection chemiluminescence method based on luminol-hydrogen peroxide system was established to determine the content of dimethoate. The results indicated that the dimethoate concentration had a good linear correlation with chemiluminescence within the scope of 0. 01 to 10 mg/L. The detection limit of the method was 0. 009 mg/L. The relative standard deviation was 2. 1% for eleven replicate detections of 0. 5 mg/L dimethoate. Using this system, the cauliflower samples were spiked with dimethoate and detected by this method with recovery ranging from 86. 7% ~109. 5%.