重寄生真菌盾壳霉与核盘菌对峙培养的差异代谢物分析

Metabolic profile of Coniothyrium minitans co-cultured with Sclerotinia sclerotiorum

运用基于液相质谱联用技术的代谢组分析手段,检测重寄生真菌盾壳霉和宿主植物病原菌核盘菌对峙培养时的代谢物差异。结果发现:盾壳霉与核盘菌对峙接触能显著诱发盾壳霉代谢物的分泌,构建盾壳霉特定代谢物数据库检测到3种代谢物涉及重寄生过程,分别为Macrosphelide A,Benzenediol和5-Aminopentanoate,且对峙接触2d时检测到的代谢物积累量最大。对盾壳霉重寄生过程代谢物变化的分析结果表明,真菌重寄生能诱发代谢物水平的交流和次级代谢物积累。

LC-MS based metabolomics analysis was used to explore metabolic profile of Coniothyri- urn rninitans co-culture with its host fungus Sclerotinia sclerotiorurn.The results showed that co-culture could significantly influence fungal metabolic profile. Three metabolites, namely Macrosphelide A, Benzenediol, and 5-Aminopentanoate, were identified in C. rninitans specific metabolites database and the largest metabolites accumulation was detected at 2 d of co-culture.This approach allowed the investiga- tion of metabolic patterns in mycoparasitism and indicated that mycoparasitism could induce metabolites communication and secondary metabolites accumulation.