3株生防真菌发酵液对大豆孢囊线虫的防治效果

Control efficiency of three fungal strains' fermentation broth on soybean cyst nematode(Heterodera glycines)

为了获得更多的防治大豆孢囊线虫的微生物资源,在室内对3株生防真菌黑曲霉NBC001、草酸青霉NBC008和草酸青霉NBC012进行大豆孢囊线虫的毒力测定和盆栽防治效果测定。结果表明,NBC001、NBC008和NBC012发酵液均有杀线虫活性,2倍稀释液处理,线虫校正死亡率约90%,孵化抑制率在68%以上。盆栽试验结果发现,NBC001、NBC008和NBC012发酵原液能使孢囊数分别降低50.07%、59.88%和57.45%;NBC001和NBC012发酵液使株高分别增加11.3%和18.6%,NBC008使大豆根长增加26.7%,表明菌株NBC001、NBC008和NBC012可用来防治大豆孢囊线虫。

In order to obtain more microorganism resources to control soybean cyst nematodes （SCN） ,control efficiency of three fungal strains including Aspergillus niger NBC001, Penicillium oxa- licum NBC008 and P. oxalicum NBC012 on soybean cyst nematodes were evaluated. In vivo analyses showed that all three fungi fermentation were lethal to nematode juveniles. The revised mortality of 2 times dilutions against SCN juveniles was about 90%, and the inhibition of egg hatchability was 68%. The pot experiment showed that cyst number treated with NBC001,NBC008, NBC012 fermentations were reduced by 50.07 %, 59.88 %, 57.45 %, respectively, compared with the control. The height of plant increased by 11.3% and 18.6% after treated with NBC001 and NBC012 fermentations, while the length of root increased by 26.7% after treated with NBC008 fermentations. The present investigation suggested that fermentations of NBC001,NBC008,NBC012 could be used to control soybean cyst nematode.