摘要
通过同源重组技术敲除克雷伯氏肺炎杆菌的luxS基因,使其群体感应系统失活,研究突变株在2,3-丁二醇发酵培养基中的生长状况及对2,3-丁二醇合成的影响;同时检测对其他副产物合成的影响,通过对野生株和luxS突变株发酵合成2,3-丁二醇的结果进行研究发现,与野生株相比,失去了群体感应功能的luxS突变株利用同样质量浓度的葡萄糖合成2,3-丁二醇的能力提高了47.75%,同时琥珀酸、乳酸和乙醇等主要副产物的质量浓度大幅降低。
The luxS gene from quorum sensing system of K. pneumoniae was deleted to study the mechanism of quorum sensing. The yield of 2,3-butanediol and several by-products in the K. pneumoniae wild type and its luxS mutant was compared. Results showed that the yield of 2,3-butanediol in the luxS mutant was 47.75 % higher than that in the production with the same concentration of glucose. The concentration of the by-products including succinic acid, lactic acid and ethanol was obviously decreased.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2017年第1期61-67,共7页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(41306142
21576279)
