葎草中生物碱提取工艺与含量测定研究

Extraction and determination of alkaloids from Humulus Scandens

目的:探讨葎草中生物碱的提取方法及含量测定。方法:采用正交实验方法,以提取温度、提取时间、料液比、盐酸浓度为影响因素,以生物碱得率为指标,优选生物碱最佳提取条件;采用生物碱颜色反应和薄层色谱进行鉴定;采用酸碱滴定方法进行生物碱含量测定。结果:葎草生物碱中含有乌头碱。葎草中生物碱的提取温度70℃、时间60 min、料液比1∶30、盐酸浓度2%为葎草中生物碱的最佳提取条件。精密度实验RSD为2.11%,加样回收试验RSD为1.46%,稳定性试验RSD为1.80%。结论:该工艺条件下葎草中生物碱的提取方法简便、快捷,生物碱得率达0.283%;精密度较高、稳定性较好、方法可行。

Objective： To discusses the extracting process and content determination of alkaloids from Humulus Scandens.Methods： Using alkaloids yield as the index, the extracting process for alkaloid was obtained by orthogonal experiment with extraction temperature, extraction time, solid-liquid ratio, and concentration of hydrochloric acid as the experimental factors. Using alkaloids color identification reaction and TLC identification to verify alkaloids. Acid-base titration was used to measure it. Results：Alkaloids in Humulus Scandens was mainly Aconitine. The best extraction conditions： the temperature of 70 ℃, time for 60 minutes, solid-liquid ratio 1∶30, and hydrochloric acid concentration of 2% for the extraction of alkaloid from Humulus Scandens.RSD was 2.11%, 1.46% and 1.80% in the precision test, the recovery test and the stability test, respectively. Conclusion： The extracting process for alkaloids from Humulus Scandens was simple and fast with high precision recovery and stability. The yield of alkaloids was 0.283%.