肝癌患者血清和组织中prohibitin的表达及其临床意义

Clinical significances and expressions of prohibitin in patients with hepatocellular carcinoma

目的研究抑制素prohibitin（PHB）在肝癌患者血清和组织中的表达及其临床意义。方法应用双抗体夹心ELISA法检测肝癌患者和健康人各82例血清PHB浓度。应用RT-PCR和免疫组织化学的方法检测PHB在30例肝癌患者组织、癌旁组织和正常肝组织中的表达水平。同时,收集肝癌患者的临床资料,分析PHB表达与各临床参数之间的相关性。结果 PHB在肝癌患者和健康人血清中浓度分别为227.97 pg/mL和55.78 pg/mL,肝癌患者血清PHB浓度明显高于健康人（Z=-4.532,P=0.000）;肝癌患者血清中的PHB浓度与ALT、TBil、AFP呈正相关（r=0.337,P=0.034;r=0.431,P=0.006;r=0.319,P=0.045）。在肝癌组织和癌旁组织中PHB蛋白阳性表达率分别为73.3%和86.7%,与正常肝组织（6.7%）比较,差异有统计学意义;在肝癌组织和癌旁组织中PHB mRNA的表达较正常组织亦明显升高;肝癌组织中PHB表达与肿瘤分化程度、TNM分期和AFP含量相关,即肿瘤低分化、TNMⅢ~Ⅳ期、AFP≥400μg/L的肿瘤组织中PHB表达明显升高,差异有统计学意义。结论 PHB在肝癌患者血清和组织中均高表达,分别与肝脏损伤的程度和HCC恶性程度相关,提示PHB可能参与HCC的发生和发展。

Objective To investigate serum and tissue expressions and the clinical significances of prohibitin in patients with hepatocellular carcinoma（HCC）.Methods Serum PHB levels in 82 HCC patients and 82 healthy individuals were determined by ELISA assay.The expression of PHB in the 30 pairs of HCC tissues,paracancerous tissues and normal tissues were detected by real-time PCR and immunohistochemistry assay.The clinical data were collected to determine the correlation between PHB and clinical features.Results Serum PHB protein level in HCC patients was significantly increased when compared with healthy individuals group（227.97 vs.55.78 pg/mL,Z=-4.532,P=0.000）.The expression of PHB in HCC patients had positive correlation with alanine aminotransferase（ALT,r=0.337,P=0.034）,total bilirubin（TBil,r=0.431,P=0.006）and AFP（r=0.319,P=0.045）.The positive rate of PHB in HCC tissues and paracancerous tissues were 73.3% and 86.7%that were significantly higher than that of the normal tissues,respectively.The PHB mRNA expression in HCC tissues and paracancerous tissues were also higher than that of the normal tissues.The expression of PHB in HCC patients had correlation with differentiation grade,TNM stage and AFP.Conclusion PHB is overexpressed in HCC patients tissues and serum and correlated with the degree of liver injury and tumor malignancy,indicating that PHB may be involved in the occurrence and progression of HCC.