瘦素对鸡原代前体脂肪细胞增殖分化的影响

Effect of Leptin on the Proliferation and Differentiation of Chicken Primary Preadipocytes

【目的】探究不同浓度瘦素对鸡原代前体脂肪细胞增殖分化的影响。【方法】以12日龄科宝肉鸡腹部脂肪为材料进行原代前体脂肪细胞培养,细胞铺板24 h后,试验组细胞以添加了0、50、200、800 ng/m L瘦素的培养基进行培养,每隔24 h换一次液,至72 h。采用CCK8法绘制对照组和瘦素处理组细胞的生长曲线,油红O提取比色法测定脂肪含量变化,Real-time PCR方法检测前体脂肪细胞分化过程中关键基因PPA R酌和SREBP1c的表达。【结果】CCK8法与油红O提取比色法表明,0～800 ng/m L瘦素对鸡前体脂肪细胞的增殖分化无显著影响（P跃0.05）,Real-timePCR结果表明不同浓度的瘦素处理对前体脂肪细胞内脂肪分化关键基因PPA R酌和SREBP1c的表达无显著影响（P跃0.05）。【结论】本试验证明0～800 ng/m L瘦素对鸡前脂肪细胞的增殖和分化无影响。

[Objective] In order to explore the effects of leptin on the proliferation and differentiation of chicken primary preadipocytes. [Methods] Separated from 12-day-old Cobb broilers abdominal fat for cell culture, cells were treated with 0, 50,200,800 ng/mL leptin after being plated for 24 h, respectively. Culture liquids were changed per 24 h time interval until 72 h. Growth curves for the four experimental groups were draw via CCK-8 method. The determination of fat content was done via oil red O extraction method. Expressions of key genes PPARγ and SREBPlc during the differentiation of preadipocytes were detected via q-PCR. [Results] The results of CCK-8 method and oil red O extraction indicated that 0-800 ng/mL leptin had no significant effect on the proliferation and differentiation of chicken preadipocytes （P〉0.05）, meanwhile the Liptin had no significant effect on the expression of PPARγ and SREBPlc （P〉0.05）, respectively. [Conclusion] The study proved that leptin had no effect on the proliferation and differentiation of chicken preadipocytes.