回乳抑增优化方对乳腺增生症伴高泌乳素血症模型大鼠的治疗作用

Effect of the Optimal Prescription of Huiru Yizeng on Hyperprolactinemia and Hyperplasia of Mammary Glands in Model Rats

目的探讨回乳抑增优化方对乳腺增生症伴高泌乳素血症大鼠模型的治疗作用。方法 Wistar雌性大鼠56只,随机分为7组,每组8只,分别为正常对照组、模型对照组、氯化钠组、溴隐亭组、乳癖散结组、原方组、优化方组。除正常对照组外,其余6组复制乳腺增生症伴高泌乳素血症大鼠模型,造模成功后灌胃给予相应药物。30 d后,测定各组大鼠体内泌乳素、孕激素和雌激素含量,观察大鼠乳腺组织病理性增生情况。结果模型对照组、原方组和优化方组泌乳素含量分别为(69.47±6.08),(53.13±10.59),(28.41±6.37)pg·m L^(-1),原方组与优化方组泌乳素含量均显著降低,但优化方组降低效果更明显(P<0.01)。原方组大鼠乳腺小叶无明显增生,个别腺泡和导管轻度增生,导管中有少量分泌物存在;优化方组乳腺小叶未见增生,导管内无分泌物,基本恢复至正常对照组水平。结论回乳抑增优化方可有效降低泌乳素含量,恢复泌乳素、雌孕激素之间平衡,改善模型大鼠乳腺病理性增生状态,药效优于原方。

Objective To investigate the effect of the optimal prescription of huiru yizeng on rats with hyperplasia of mammary gland and hyperprolactinemia. Methods Fifty-six female Wistar rats were randomly divided into 7 groups（ n = 8）,including normal control group,model control group,sodium chloride group,bromocriptin group,rupi sanjie group,the original prescription group and optimizing prescription group. Rat model of mammary gland hyperplasia with hyperprolactinemia was replicated in 6 groups but not the normal control group. The successfully established experimental rats were given corresponding drugs by intragastric gavage. After 30 days,the levels of the estradiol,progesterone,and prolactin were detected,and the pathomrphology of glandular tissue was observed. Results Prolactin levels of model control group,the original prescription group and optimize prescription group were（ 69. 47 ± 6. 08）,（ 53. 13 ± 10. 59）,and（ 28. 41 ± 6. 37） pg·mL^-1,respectively.Compared with that in the model control group,the contents of prolactin in both the optimal prescription group and the original prescription group were reduced,but the optimal prescription group was better（ P〈0.01）. In the original prescription group,the lobules of mammary gland showed a few of hyperplasia,the individual alveoli and duct showed a slight hyperplasia,and a small amount of secretions was found in the duct. The degree of the hyperplasia was alleviated in the optimal prescription group similar to that observed in the normal control group,which showed that there was no hyperplasia in the lobules of mammary gland or no secretions in the duct. Conclusion The therapeutic effects of the optimal prescription are much better than the original prescription,which can effectively lower the level of prolactin, adjust the balance among the prolactin,estrogen and progesterone,and alleviate the pathological hyperplasia of mammary glands in the model rats.