高粱查尔酮合成酶的生物信息学分析

Bioinformatics analysis of chalcone synthase in Sorghum bicolor

为研究高粱查尔酮合成酶（CHS）基因的功能,利用生物信息学工具和方法,对CHS蛋白质理化性质、疏水性/亲水性、跨膜区域、结构功能域、二级结构、三级结构和同源性进行了预测与分析。结果显示,高粱的CHS蛋白质共有8种（CHS1-CHS8）,其氨基酸数量都在400左右,分子量差别不大;其中CHS1-CHS7为较稳定的亲水性蛋白质,CHS8为不稳定的疏水性蛋白质;8种蛋白质都为非跨膜蛋白。CHS蛋白有Chal_sti_synt_N和ACP_syn_lll_C 2个结构域,主要构件为α-螺旋和不规则卷曲。同源性分析结果表明,CHS1~CHS7蛋白质氨基酸序列间的同源性较高。

To obtain more information about chalcone synthase （ CHS） gene from Sorghum bicolor,the bioinformatics methods and tools were used to analysis the physical and chemical properties,hydrophobic/hydrophilic,transmenbranne re-gion, structure and function domain,secondary structure,tertiary structure and homology of CHS protein in Sorghum bicolor. The results showed that there were eight CHS proteins in Sorghum bicolor （CHS1-CHS8）. The number of amino acids was a-bout 400 in CHS proteins. All CHS proteins were stable and hydrophilic protein except for CHS8. The analysis also demon-strated that the eight CHS proteins were non-transmembrane protein. Its main component was alpha-helix and random coil. The structure domains of CHS proteins consisted of Chal_sti_synt_N and ACP_syn_lll_C. The sequence homology was higher in CHS1-CHS7 proteins.