天山雪莲水孔蛋白基因(AQP)家族鉴定与生物信息学分析

Identification and bioinformatics analysis of aquaporin gene(AQP) family in Saussurea involucrate Kar.

为研究新疆天山雪莲水孔蛋白基因(AQP)家族并对其基因家族成员进行鉴定及分类,从雪莲转录组数据库中获得了33条天山雪莲水孔蛋白基因序列,利用生物信息学软件,将其与拟南芥中38条水孔蛋白基因序列进行分析、分类和保守序列分析,结果显示天山雪莲33条AQP基因家族序列与拟南芥38条AQP基因序列一样分成4个亚族,即质膜水孔蛋白(Plasma membrane intrinsic protein,PIP)基因、液泡膜内在水孔蛋白(Tonoplast intrinsic protein,TIP)基因、类Nodulin(NOD26)膜内在水孔蛋白(NOD26-like membrane intrinsic protein,NIP)基因和膜内小基本蛋白(Small and basic membrane intrinsic protein,SIP)基因。拟南芥与天山雪莲AQP基因间具有较高的保守性。对天山雪莲AQP基因家族进行了系统进化树构建,并分析了水孔蛋白氨基酸组成成分、理化性质,预测了二级结构,发现水孔蛋白不同亚族间氨基酸数目、氨基酸序列间的亲水性存在一定差异,水孔蛋白的二级结构以α螺旋、β折叠、无规则卷曲为主。从这33条AQP基因序列中克隆了3个水孔蛋白基因,测序结果证明天山雪莲转录组数据库基因序列准确率较高。

To identify the aquaporin （ AQP） gene family of Saussurea involucrate Kar. ,33 sequences of the AQP were acquired from transcriptome database. Using a variety of bioinformatics softwares,the gene sequences of S. involucrate Kar. AQP and Arabidopsis thaliana AQP were analyzed and classified. 33 S. involucrate Kar. AQP genes as well as 38 Arabidopsis AQP genes were classified into four subfamilies,plasma membrane intrinsic protein gene（ PIP ）,tonoplast intrinsic protein gene （ TIP）, NOD26-like membrane intrinsic protein gene（ N IP ） and small and basic membrane intrinsic protein gene（ S IP ） . AQP genes were highly conservative between Arabidopsis and S. involucrate Kar. . There were some differences in the number of amino acids and the hydrophilicity of amino acid sequence among different subfamilies of AQP gene. The secondary structure of AQP protein were dominated by alpha helix, beta bridge, and random coil. The sequencing of three AQP gene cloned from 33 AQPgene sequences verified the accuracy of gene se-quences in transcriptome database.