盐城地方鸡MSTN基因外显子1多态性及其与生产性能的相关性分析

Association of MSTN gene with growth and reproductive traits in Yancheng chicken

本研究以MSTN基因作为影响鸡生长性状和繁殖性状的候选基因,采用DNA测序和PCR-SSCP技术分析MSTN基因单核苷酸多态性,并与鸡的生产性能进行相关性分析。结果显示,共发现2个SNP位点(G2100A和G2283A),其中G2283A位点DD基因型16周龄的体质量极显著高于CC基因型(P<0.01),并显著高于CD基因型(P<0.05),CC基因型300日龄产蛋数极显著的高于DD基因型(P<0.05)。G2100A位点AA和AB基因型的开产日龄显著早于BB基因型(P<0.05)。对基因型组合进行分析,发现AA×DD基因型组合16周龄体质量、20周龄体质量、300日龄体质量最高,可作为影响鸡生长性状的优秀基因型组合。

In this research, PCR-SSCP and DNA sequencing were used to detect the SNPs of MSTN gene in order to analyse their relationships with production traits in chicken. The results showed that two SNPs loci （ G2100A and G2283A） were found. The body weight in DD genotype of G2283A was significantly higher than that of CC （ P〈0. 01 ）and CD geno-types （P〈0. 05） at 16 weeks of age,and the number of eggs in CC genotype was significantly higher than that of the DD （P〈0. 05） at 300 days of age. The chickens in AA and AB genotypes of G2100A presented significant lower age at first egg than that of BB genotype （ P〈0. 05）. Combination of AA and DD genotypes can be considered as the dominant factors af-fecting chicken growth traits.