不同血细胞分离机采集程序对健康供者造血干细胞采集物细胞成分的影响

Effect of Different Hemapheresis Procedures on Components of Hematopoietic Stem Cells Collected from Healthy Donors

目的:观察COBE Spectra血细胞分离机AutoPBSC程序以及Spectra Optia血细胞分离机MNC程序对采集的健康供者外周血造血干细胞成分的影响。方法:2015年1月至2016年8月期间对12例供者随机应用COBE Spectra血细胞分离机AutoPBSC程序以及Spectra Optia血细胞分离机MNC程序进行外周血造血干细胞采集,观察应用不同采集程序获得的采集物中单个核细胞、CD34^+细胞、粒细胞、淋巴细胞、血小板数的差异。结果:两种采集程序在循环血量、采集时间及抗凝剂使用量方面无显著差异。COBE Spectra血细胞分离机AutoPBSC程序采集的采集物体积及单个核细胞计数均低于Spectra Optia血细胞分离机MNC程序的采集物体积及单个核细胞计数。AutoPBSC程序采集的CD34^+细胞计数高于MNC程序采集的CD34^+细胞计数。AutoPBSC程序采集物中混入的淋巴细胞及血小板较MNC程序中多,其差异均有统计学意义(P<0.05)。结论:与Spectra Optia血细胞分离机MNC程序相比,COBE Spectra血细胞分离机AutoPBSC程序采集的造血干细胞数量多,混入的淋巴细胞及血小板多。

Objective：To investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells（HSCs） collected from helathy donors.Methods：twelve donors were underwent stem cell collection from January 2015 to August 2016,and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator,the mononuclear cells,CD34^＋ cells,granulocytes,lymphocytes and platelets in the collections were compared.Results：The circulating blood volume,the acquisition time and dosage of anticoagulants were not significantly different between two procedures.The volume and the mononuclear cell count collected by AutoPBSC procedure were lower than those by the MNC procedure,while the CD34^＋ cell count by AutoPBSC procedure was higher than that by the MNC procedure.More lymphocytes and platelets were collected by AutoPBSC procedure as compared with that by the MNC procedure（P〈0.05）.Conclusion：Compared with MNC procedure of the Spectra Optia blood cell separator,the number of collected stem cells,lymphocytes and platelets are higher by using AutoPBSC procedure of the COBE spectra blood cell separator.