不同贮存时间的单采少白细胞血小板中精氨酸酶水平的研究

Arginase Level in Apheresis Leukocyte-Reduced Platelets Stored for Different Time

目的:探讨不同贮存时间对单采少白细胞血小板(apheresis leukocyte-reduced platelet,ALR-Plt)中精氨酸酶水平的影响及精氨酸酶的可能来源。方法:应用ELISA法检测不同贮存时间ALR-Plt和对照血浆中精氨酸酶水平及髓过氧化物酶(myeloperoxidase,MPO)水平;应用相关性分析分析不同贮存时间的ALR-Plt中精氨酸酶水平与MPO水平的相关性。结果:贮存3 d内的ALR-Plt与对照血浆中精氨酸酶水平差异无统计学意义,但是贮存超过3d的ALR-Plt中精氨酸酶水平显著高于贮存3 d内的ALR-Plt和对照血浆中的水平(P<0.05)。不同贮存时间的ALR-Plt之间MPO水平差异无统计学意义,但均显著低于对照血浆中的水平(P<0.05)。相关性分析显示:ALRPlt中精氨酸酶水平与MPO水平呈正相关(r=0.58)。结论:贮存时间超过3 d的ALR-Plt中精氨酸酶水平显著升高,其可能来源于残留的少量白细胞,特别是中性粒细胞。

Objective：To explore the effect of storage time on arginase level and possible source of arginase in apheresis leukocyte-reduced platelets（ALR-P1t）.Methods：The arginase level and myeloperoxidase（MPO） levels in ALR-Plt and control plasma were detected by ELISA.The relationship between arginase level and MPO level in ALR-Plt was analyzed by correlation analysis.Results：There was no significant difference of arginase level between ALR-Plt stored less than 3 days and control plasma.However,arginase level in ALR-Plt stored over 3 days was significantly higher than that in ALR-Plt stored less than 3 days and control plasma（P〈0.05）.There was no significant difference of MPO level in ALR-Plt stored for different times,but the MPO level in ALR-Plt stored for different time was lower than that in control plasma.Correlation analysis showed that arginase level positively correlated with MPO level in ALR-Plt of different storage time（r =0.58）.Conclusion：The arginase level in ALR-Plt stored over 3 days increase significantly.The main possible source of arginase in ALR-Plt is the residual white blood cells,especially neutrophils.