体外培养肌源性干细胞向造血方向分化的实验研究进展

Progress of Experimental Research on Differentiation of Muscle-Derived Stem Cells into Haematopoietic Lineages in Vitro

肌源性干细胞(muscle-derived stem cell,MDSC)是在肌肉中发现的多功能干细胞。因其来源丰富,取材便捷和体外移植存活率高等特点,因而在血液病的治疗中MDSC展示出良好的应用前景。研究人员对MDSC进行体外培养时,在取材鼠的选择、MDSC分离和纯化等实验方法方面存在较大差异。此外,促进MDSC体外增殖并向造血细胞分化的培养条件以及相关作用机制还不清晰。本文主要就体外培养MDSC向造血方向转化的实验研究现状作一综述,讨论的具体问题包括MDSC的体外培养,MDSC的鉴定,MDSC的增殖和MDSC向造血方向的分化等。

Muscle-derived stem cells（MDSC） are a population of multipotent stem cells in the muscular tissue.It provide an excellent prospect of hemopathy treatment due to their superiorities,such as rich sources,convenient material resource and a high survival rate after transplantation and so on.However,there are great differences in sampling,separation,purification,and proliferation when MDSC were cultured in vitro.In addition,the proliferation conditions of the MDSC in vitro are yet unclear.The related regulatory mechanisms,which MDSC transformed into haematopoietic cells,need to be investigated.In this article,the experimental researches on the differentiation of MDSC into haematopoietic lineages are reviewed,the concrete problems discussed in this review are culture of MDSC in vitro,identification of MDSC,proleferation of MDSC,differention of MDSC in to hematopoietic lineages and so on.