亚硝基胍与紫外诱变选育高效解钾菌株

Screening of High Efficiency Potash Feldspar-Solubilizing Bacteria by NTG/UV

为构建遗传背景差异较大、解钾性能稳定提高的候选菌库,选用课题组分离的蜡样芽孢杆菌L18(Bacillus cereus,KF494191)为出发菌株,对其分别进行亚硝基胍(NTG)和紫外(UV)诱变处理,经四苯硼钠-季铵盐显色初筛,摇瓶发酵复筛,获得42株解钾能力显著增强的突变株,其中:诱变株L18-NTG-151和L18-UV-68发酵液钾质量浓度分别为126.74,121.69mg/L,与出发菌株相比分别提高了63.64%和57.12%.结果表明亚硝基胍和紫外线诱变处理能显著提高蜡样芽胞杆菌的解钾能力.

In order to build a bacteria library with large difference genetic background and the Potassiumsolubilizing performance,an original Silicate bacteria L18（Bacillus cereus,KF494191）was selected.It was induced mutagenesis by nitrosoguanidine（NTG）culturing and ultraviolet（UV）radiating.42 mutant bacteria with obviousincreased potassium-solubilizing performance were screened from a large number of mutant bacteria.The K content in fermentation of 2 mutant bacteria（L18-NTG-151,L18-UV-68）were126.74mg/L and 121.69 mg/L,which were improved by 63.64% and 57.12% compared with original bacteria L18（77.45mg/L）.The results show thatboth two methods of NTG or UV can significantly improve the potassium-solubilizing capacity of Bacillus cereus.