氧化苦参碱联合5-FU对人胃癌SGC-7901细胞生长的协同抑制作用及其机制研究

Effect of combination Oxymatrine with 5-FU inhibited proliferation in SGC-7901 human gastric cancer cells and its mechanism

目的:氧化苦参碱联合5-FU对人胃癌SGC-7901细胞生长的协同抑制作用及相关机制研究。方法:通过不同浓度的氧化苦参碱单独及联合应用5-FU作用于SGC-7901细胞24、48、72 h,采用MTT法检测细胞活力,HOECHST染色法检测细胞凋亡,免疫细胞法检测胃癌SGC-7901细胞内血管内皮生长因子(VEGF)蛋白的表达,以逆转录聚合酶链式反应RTPCR法观察SGC-7901细胞VEGF mRNA的转录情况。结果:与空白组比,氧化苦参碱中剂量、高剂量组及其联合5-FU组均能显著抑制人胃癌SGC-7901细胞生长,并诱导其凋亡(P<0.01);与单独使用5-FU组相比,联合用药组细胞增殖抑制率和凋亡率均显著增高(P<0.05);氧化苦参碱及联合5-FU组中人胃癌SGC-7901细胞的VEGF mRNA转录及其蛋白表达显著降低(P<0.01)。结论:氧化苦参碱对5-FU的胃癌SGC-7901细胞的增殖抑制和诱导凋亡具有协同作用;促进氧化苦参碱对VEGF的基因和蛋白表达抑制作用可能是其机制之一。

Objective： To study the synergy inhibition effects of Oxymatrine combine with 5-FU on proliferation and apoptosis of SGC7901 human gastric cancer cells and its mechanism. Methods： SGC7901 cells were treated by different concentrations of Oxymatrine with or without 10. 0 mg / L 5-FU for 24,48 and 72 h. The cells proliferative vitality was detected by MTT assay,and the apoptosis of SGC7901 cells was detected by HOECHST stain. Immunohistochemistry was applied to examine the protein expression of VEGF. The transcriptions of VEGF mRNA were distinguished by RT-PCR technique. Results： Compared with control group,middle dose and high dose concentration of Oxymatrine groups,and groups of its combined with 5-FU can inhibit the proliferation and induced the apoptosis of SGC7901 cancer cells. Compared with 5-FU group,the inhibition rate and apoptosis rate were significantly increased in groups of Oxymatrine combined with 5-FU. The mRNA and protein expression of VEGF were significantly decreased in groups of Oxymatrine and it combined with 5-FU. Conclusion： Synergy inhibition effects of Oxymatrine combine with 5-FU on proliferation and apoptosis of SGC7901 human gastric cancer cells were found,which may be related to strengthen the effects of Oxymatrine of inhibiting angiogenesis relate with down-regulating the expression of VEGF.