痹痛灵胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量

Determination of ginsenoside Rg1 and Rb1,notoginsenoside R1 in Tong Biling Capsule by HPLC

目的：采用高效液相色谱法（HPLC）测定痹痛灵胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量。方法：色谱条件：以十八烷基硅烷键合硅胶为填充剂，以乙腈为流动相A，以水为流动相B，柱压11．0mPa，流速：1．0mL／min，柱温：45℃，检测波长为203nm，理论板数按三七皂苷R1峰计算应不低于4000。拖尾因子为1．03。结果：本测定法精密度、重复性、稳定性、回收率良好。人参皂苷Rgl进样量在0．8016—8．0160μg，Rb1在0．8320—8．3200μg，三七皂苷R1在0．2000—2．0000μg内皆与峰面积呈良好的线性关系。结论：本法简便、灵敏、准确、精密度、重复性、稳定性、回收率良好，可有效的控制痹痛灵胶囊的质量。

Objective： To determine the content of ginsenoside Rgl, Rb1 and 37 saponins R1 in Bi Tong Ling Capsule by high performance liquid ehro- matngraphy （HPLC）. Methods： The chromatographic conditions： Eighteen alkyl silane bonded silica as filler, with acetonitrile as mobile phase A, with water as mobile phase B, pressure 11. 0mPa, flow rate： 1. 0mL/min, column temperature： 45 degrees centigrade, the detection wavelength was 203nm, the number of theoretical plates should not be less than 4000 calculated by 37 sapenins R1 peak. The tailing factor is 1. 03. Results： The precision, re- peatability, stability and recovery of the method were good. Ginsenoside Rgl injection in 0. 8016 ~ 8. 0160g, Rb1 in 0. 8320 ~ 8. 3200 μg, 37 saponin RI in 0. 2000 ~ 2 μg in the peak area is good linear relationship. Conclusion： This method is simple, sensitive, accurate, precise, reproducible, stable and good recovery rate. It can effectively control the quality of the capsule.