急性髓系白血病患者NKD1基因的表达及其临床意义

Expression of NKD1 and its clinical significance in acute myeloid leukemia

目的:检测急性髓系白血病(acute myeloid leukemia,AML)患者裸角质膜同源蛋白(NKD1)的表达水平并分析其临床意义。方法:应用实时定量PCR方法检测125例初诊AML患者和22例对照者骨髓单个核细胞中NKD1的转录水平。根据NKD1表达水平将AML患者分为高、低表达两组,比较两组间临床参数的异同并分析其临床意义。结果:AML患者中NKD1的表达水平较对照组明显降低(U=805,P=0.002)。NKD1高、低表达两组患者的性别、年龄、外周血白细胞计数、外周血血小板计数、骨髓原始细胞比例、FAB/WHO分型以及常见基因突变间的差异无统计学意义(P均>0.05);NKD1高表达组血红蛋白水平明显高于低表达组(U=1 339,P=0.029);NKD1高、低表达两组患者经诱导治疗后完全缓解率间的,差异无统计学意义(P>0.05);全部AML、非M3及染色体核型正常患者中,NKD1低表达组总体生存时间明显短于NKD1高表达组(P均<0.05)。结论:NKD1在AML患者中呈低表达,可作为判断AML患者预后的分子标志物。

Objective：To investigate the expression of NKD1 in patients with acute myeloid leukemia （AML）and analyze its clinical significance.Methods：Real-time quantitative PCR was performed to detect NKD1 mRNA level in the bone marrow mononuclear cells from 125 de novo AML patients and 22 controls. Clinical significance of NKD1 expression was analyzed by comparing the clinical parameters in the two groups.Results：NKD1 expression in AML patients was significantly lower than controls（U =805,P =0.002）.There were no significant differences in sex,age,blood parameters,bone marrow cell percenta-ges,platelets counting,FAB /WHO subtypes and ten genes mutations between the patients with high-ex-pression and low-expression NKD1（both P 〉0.05）.However,NKD1 high-expressed patients were showed to have higher levels of hemoglobin than low-expression patients（U =1 339,P =0.029）.After the induc-tion therapy on the patients,there was no significant difference in complete remission rate between the two group patients （P 〉0.05）.However,the low-expression NKD1 group had shorter survival time than the group with high-expression NKD1 group in all AML,non-M3 AML and cytogenetically normal AML patients （both P 〈0.05）.Conclusion：AML patients showed low-expression NKD1,which could be used as a mo-lecular marker for predicting prognosis in de novo AML patients.