猪胰高血糖素样肽-2通过细胞外信号调节激酶1/2调节仔猪空肠上皮细胞紧密连接蛋白表达

Porcine Glucagon-Like Peptide-2 Regulates Tight Junction Protein Expressions in Porcine Small Intestinal Epithelial Cell through Extracellular Regulated Kinase 1/2 Signaling Pathway

本试验以体外培养的仔猪空肠上皮细胞(IPEC-J2)为研究对象,研究了猪胰高血糖素样肽-2(p GLP-2)对紧密连接蛋白表达的调节及其信号传导机制。培养液中分别添加10-9mol/L p GLP-2(p GLP-2组)和10-9mol/L p GLP-2、10μmol/L U0126(p GLP-2+U0126组),对照组不添加以上试剂,每组3个重复,每个重复1个培养孔。测定IPEC-J2胞质紧密黏连蛋白-1(ZO-1)、occludin、claudin-1以及细胞外信号调节激酶1/2(ERK1/2)蛋白表达量。结果表明:与对照组相比,IPEC-J2细胞培养液中添加p GLP-2显著增加了ZO-1、occludin、claudin-1以及p42-ERK1/2、p44-ERK1/2的蛋白表达量(P<0.05);与p GLP-2组相比,在IPEC-J2细胞培养液中加入ERK1/2的抑制剂U0126,显著降低了ZO-1、occludin、claudin-1以及p42-ERK1/2、p44-ERK1/2的蛋白表达量(P<0.05)。综合得出,ERK1/2通路是p GLP-2调控肠道上皮细胞紧密连接蛋白表达的一条重要信号通路。

Porcine small intestinal epithelial cell from jejunum（IPEC-J2） was used to study the regulation of porcine glucagon-like peptide-2（pGLP-2） on protein expressions of tight junction and its signaling pathway.Culture mediums were supplemented without（control group） or with 10^-9 mol/L pGLP-2（pGLP-2 group）,and10^-9 mol/L pGLP-2 and 10 μmol/L U0126（pGLP-2＋U0126 group）,respectively.Each group had 3 replicates with 1 culture pore per replicate.Protein expressions of zonula occludens-1（ZO-1）,occluding,claudin-1 and extracellular regulated kinase 1/2（ERK1/2） in cytoplasm of IPEC-J2 were determined.The results showed as follows：compared with control group,the supplementation of pGLP-2 in culture medium of IPEC-J2 significantly increased protein expressions of ZO-1,claudin-1,occludin,p42-ERK1/2 and p44-ERK1/2（P〈0.05）;compared with pGLP-2 group,the supplementation of U0126,an inhibitor of ERK1/2,in culture medium of IPECJ2 significantly decreased protein expressions of the above proteins（P〈0.05）.The results suggest that ERK1/2pathway is an important signaling pathway of pGLP-2 regulating tight junction protein expressions in intestinal epithelial cells.