驱蚊帐药效实验研究

Study on the residual bio-efficacy of long-lasting insecticide-treated nets for mosquito control

目的检测驱蚊帐驱、灭蚊效果。方法按NY/T 1151.4-2012《农药登记卫生用杀虫剂室内药效试验及评价第4部分驱蚊帐》开展驱蚊帐室内接触筒药效检测和模拟现场药效检测,对经不同洗涤次数的驱蚊帐的药效结果用SPSS11.5进行χ2检验和相关性分析。结果实验室接触筒实验中,样品1在经0、10、20次洗涤后,对致倦库蚊的1 h击倒率为77.7%、28.8%和0,24 h致死率为57.9%、17.2%、0;样品2则为91.8%、90.4%、74.7%和88.1%、80.3%、29.4%,不同洗涤次数间驱蚊帐对致倦库蚊杀灭效果的差异均有统计学意义(P<0.05)。模拟现场实验中,样品1和样品2经洗涤20次后对致倦库蚊的12 h击倒率分别为57.7%和29.9%,24 h死亡率分别为57.7%和29.9%。结论洗涤影响驱蚊帐的驱灭蚊效果,实验方法的统一有利于对驱蚊帐产品开展效果评价。

Objective To assess the efficacy of long-lasting insecticide-treated nets against mosquitoes using the standard protocal. Methods The forced-contact test and analogous site test were conducted according to the standard of The Ministry of Agriculture： Laboratory Efficacy Test Methods and Criteria of Public Health Insecticides for Pesticide Registration,Part 4： Long-lasting Insecticide Treated Mosquito Net（ NY / T 1151. 4-2012）. Efficacy difference in washing groups was determined using a chi-square test and correlate analysis in SPSS 11. 5. Results In the forced-contact test,knockdown rates against mosquitoes for sample 1 after 0,10,20 times of washing within 60 minutes were 77. 7%,28. 8% and 0 respectively,and the mortality within 24 h were 57. 9%,17. 2% and 0respectively. Knockdown rates of mosquitoes for sample 2 after 0,10,20 washing times within 60 minutes were91. 8%,90. 4% and 74. 7% respectively,and mortality within 24 h were 88. 1%,80. 3% and 29. 4%respectively. There was statistical difference among different washing groups（ P 〈 0. 05）. In the analogous site test,knockdown rates within 12 h for sample 1 and sample 2 were 57. 7% and 29. 9% respectively. Mortalities within 24 h for sample 1 and 2 were 57. 7% and 29. 9% respectively. Conclusion Washing had significant effect on the longlasting insecticidal mosquito nets. The consistency of experimental methods could enhance the accuracy of assessment results of this kind of product.