热休克基因相关蛋白在大鼠牙髓增龄性变化中的表达

Age-related changes of heat shock protein expression in the dental pulp of SD rats

目的:探讨热休克基因相关蛋白在牙髓组织增龄性变化过程中的表达。方法:取雄性SD大鼠30只,其中月龄>10个月为老龄组(n=15),<2个月为年青组(n=15),然后取两组大鼠下颌第一磨牙连续切片及牙髓组织,用免疫组化染色,RT-PCR、Western Blot检测比较两组大鼠牙髓组织中热休克转录因子1(HSF1)、热休克蛋白27(HSP27)的表达水平。结果:HSF1和HSP27均表达于SD大鼠牙髓组织中,且HSF1mRNA及蛋白表达水平随大鼠年龄的增加而升高,而其下游的HSP27表达水平却随之下降。结论:热休克基因相关蛋白可能参与牙髓增龄性变化的过程。

AIM ： To investigate the age - related changes of heat shock protein（HSP） expression in dental pulp tissue. METHODS： Male SD rats with the ages of 〉 10 m （n = 15） and 〈 2 m （n = 15） were used in this study. The mandibles with the bilateral first molars were sectioned, fixed and decalcified. The expression of heat shock transcription factor 1 （ HSF1 ） and heat shock protein 27 （ HSP27 ） in the dental pulp tissues were examined by immu- nohistochemical staining, RT-PCR and Western blot. RESULTS： Both HSF1 and HSP27 were expressed in the dental pulp tissues of SD rats. The mRNA and protein expression of HSF1 increased with age. However, the mRNA and pro- tein expression of HSP27 decreased with age. CONCLUSION： Heat shock protein might be involved in the process of dental pulp ageing.