清瘟败毒饮治疗急性腹膜炎的动物实验研究

Experimental study of Qingwen Baiduyin for the treatment of acute peritonitis

目的探讨清瘟败毒饮在治疗胃肠穿孔急性腹膜炎和脓毒血症方面的疗效。方法将60只SD大鼠随机分为对照组、盲肠结扎穿孔组(CLP模型组)、清瘟败毒饮低剂量组及高剂量组。在清瘟败毒饮干预不同时间点(12、24、36及48 h),测定大鼠直肠温度、外周血白细胞(WBC)计数和血清C反应蛋白(CRP);同时,采用酶联免疫吸附测定(ELISA)法进行白细胞介素-8(IL-8)、白细胞介素-10(IL-10)及肿瘤坏死因子α(TNF-α)检测。结果与盲肠结扎穿孔组比较,清瘟败毒饮组可显著降低动物体温和白细胞数量(P<0.05),也可显著降低肿瘤坏死因子α和白细胞介素-8水平(P<0.05),并明显升高白细胞介素-10水平,有明显的统计学差异(P<0.05)。与对照组比较,盲肠结扎穿孔组C反应蛋白明显增加,给予清瘟败毒饮干预后,C反应蛋白呈下降趋势。结论清瘟败毒饮对急性腹膜炎和脓毒血症具有良好抗炎作用。

Objective To investigate the clinical therapeutically effects of Qingwen Baiduyin on acute peritonitis and sepsis caused by gastrointestinal perforation.Methods 60 adult SD rats were randomly divided into four groups,including control,CLP group,Qingwen Baiduyin low and high dose group.After the treatment of Qingwen Baidnyin for indicated time, WBC and temperature were observed; CRP, IL-8, IL- 10 and TNF-a were detected with ELISA, respectively. Results Compare with CLP group, Qingwen Baiduyin significantly decreased WBC and temperature in experimental rats（P〈0.05） , and as well as the level of TNF-α and IL-8（P〈0.05） ,however notably increased the level of IL-10（P〈0.05）.Compare with control group,the level of CRP increased significantly in CLP group,however CRP was shown on downward trend after the treatment with Qingwen Baiduyin.Conclusion Qingwen Baiduyin had a good anti-inflammatopy effect on acute peritonitis and sepsis.