TRAF6在胃癌患者腹直肌中的表达及意义

Expression and significance of TRAF6 in rectus abdominis of gastric cancer patients

目的：分别检测各期胃癌患者和腹腔良性疾病患者腹直肌中肿瘤坏死因子受体相关因子6（TRAF6）的表达情况，分析其表达的差异性，并进一步探讨 TRAF6与胃癌患者营养状况及预后的关系。方法应用实时荧光定量 PCR 和 Western blotting 检测 TRAF6在64例各期胃癌患者和53例腹腔良性疾病患者腹直肌中的表达情况，并与患者临床数据相结合，探讨其表达的意义。结果与腹腔良性疾病患者相比（1．249±0．723），Ⅰ期（2．080±0．611）、Ⅱ期（2．126±0．640）、Ⅲ期（3．127±0．953）、Ⅳ期（3．289±0．734）胃癌患者腹直肌中 TRAF6 mRNA 的表达显著升高，差异有统计学意义（t ＝4．107，P ＝0．001；t ＝4．128，P ＝0．000；t ＝8．741，P ＝0．000；t ＝9．208，P ＝0．000）。与腹腔良性疾病患者相比（0．088±0．013），Ⅰ期（0．198±0．042）、Ⅱ期（0．209±0．051）、Ⅲ期（0．295±0．072）、Ⅳ期（0．345±0．084）胃癌患者腹直肌中 TRAF6蛋白的表达显著升高，差异有统计学意义（t ＝5．203，P ＝0．000；t ＝5．642，P ＝0．000；t ＝9．351，P ＝0．000；t ＝9．854，P ＝0．000）。TRAF6 mRNA 在67．2％（43／64）的胃癌患者腹直肌组织中呈高表达。TRAF6 mRNA 的表达与胃癌患者体质量下降百分比（χ^2＝12．231，P ＝0．000）、白蛋白水平（χ^2＝22．808，P ＝0．000）显著相关。TRAF6 mRNA 在胃癌患者腹直肌中的表达与生存率显著相关（χ^2＝3．933，P ＝0．047）。结论TRAF6在胃癌患者腹直肌中呈高表达，可能在胃癌患者恶病质的发生、发展过程中发挥关键性作用，其高表达提示预后不良。

Objective To research the expression of tumor necrosis factor receptor-associated factor 6 （TRAF6）in rectus abdominis of gastric cancer patients and abdominal benign disease patients,and discuss the relationship between nutritional status and prognosis of gastric cancer patients with TRAF6.Methods Biopsies of the rectus abdominis were obtained intraoperatively from 64 gastric cancer patients and 53 subjects undergoing surgery for benign abdominal diseases.The expression levels of TRAF6 mRNA and protein were assessed through quantitative real time reverse transcriptase PCR （RT-QPCR）and Western blotting,and the relationships between nutritional status and prognosis of gastric cancer patients and the expression status of TRAF6 were analyzed. Results TRAF6 mRNA was significantly upregulated in muscle of stageⅠ（2.080 ±0.611 ）,stage Ⅱ（2.126 ± 0.640）,stage Ⅲ （3.127 ±0.953）,stage Ⅳ （3.289 ±0.734）gastric cancer compared with the control group （1 .249 ±0.723）,t =4.107,P =0.001 ;t =4.128,P =0.000;t =8.741 ,P =0.000;t =9.208,P =0.000. TRAF6 protein was significantly upregulated in muscle of stageⅠ（0.198 ±0.042）,stage Ⅱ（0.209 ±0.051 ）, stage Ⅲ （0.295 ±0.072）,stage Ⅳ （0.345 ±0.084）gastric cancer compared with the control group （0.088 ± 0.01 3）,t =5.203,P =0.000;t =5.642,P =0.000;t =9.351 ,P =0.000;t =9.854,P =0.000.TRAF6 was upregulated in 67.2% （43 /64）of gastric cancer patients.The expression of TRAF6 in muscles of gastric cancer was associated with the percent of weight loss （χ^2 =12.231 ,P =0.000）,the level of serum albumin （χ^2 =22.808,P =0.000）and the survival rate （χ^2 =3.933,P =0.047）.Conclusion TRAF6 is significantly upregulated in rectus abdominis of gastric cancer,suggesting that TRAF6 may play an important role in progres-sion and prognosis of cancer cachexia,and its overexpression suggests unfavorable prognosis.