大黄素对大鼠肾纤维化肾间质中血小板源性生长因子(PDGF-B)表达的影响及意义

The effect and significance of emodin on expression of tissue inhibitor of platelet derived growth factors-B (PDGF-B) in rat with renal tubulointer-stitial fibrosis

目的探讨大黄素对大鼠肾间质纤维化模型中血小板源性生长因子(PDGF-B)表达的影响及意义。方法清洁级Wister雄性成年大鼠160只随机分为4组:正常对照组、假手术组、模型组及大黄素治疗组。制备单侧输尿管梗阻(UUO)模型[1];治疗组在UUO的基础上每天以大黄素40mg/kg灌胃,分别在术后1d、3d、7d、14d,每组取10只大鼠处死,取左侧肾脏行免疫组织化学方法检测肾组织中PDGF-B的表达水平,动态观察PDGF-B在各组大鼠术后梗阻侧肾脏1、3、7、14天的组织学变化及大黄素对其影响。结果模型组PDGF-B的表达持续升高;治疗组PDGF-B的表达水平低于模型组(P<0.01)。结论中药大黄素可以通过减少PDGF-B的表达来发挥其延缓肾间质纤维化进程的作用,对肾纤维化有治疗作用。

Objective：To investigate the effect and significance of emodin on expression of tissue inhibitor of platelet derived growth factors-B（PDGF-B）in rat with renal tubulointer-stitial fibrosis. Methods：A total of 160 healthy Wister male rats were randomly divided into 4 groups：the normal control group,the sham-operatedgroup,the UUO group and the emodin treated group（DTG）. Rat in the DTG were administered intra-gastrically with emodin 40mg/kg daily after modeling.Ten rats of each group were sacrificed respectively at 1,3,7 and 14 days after UUO. Renal histopathological changes was observed by light microscope.The expression of PDGF-B was examined by immunohistochemistry at each time point. Results：In UUO group,the express of PDGF-B in renal interstitium was increased gradually. In DTG group,the express of PDGF-B decreased significantly compared to the same time in the UUO group（P〈0.01）. Conclusions：Emodin can delay the progression of renal tubulointerstitial fibrosis and protect the function of renal tubule probably by inhibiting the expression of PDGF-B.