香菇多糖体外逆转A2780卵巢癌细胞顺铂耐药及可能机制

Reversion of Lentinan on A2780 Ovarian Cancer Cell′s Resistance to Cisplatin in vitro and Mechanism

目的:研究香菇多糖体外逆转A2780卵巢癌细胞顺铂耐药及可能机制。方法:根据药物处理方法将A2780卵巢癌细胞分为CON组(未行香菇多糖或顺铂处理)、DDP组(仅用顺铂处理)、LEN组(仅用香菇多糖处理)及D+L组(香菇多糖联合顺铂处理),比较四组细胞增殖、细胞周期及凋亡率和耐药基因mRNA表达的差异。结果:D+L组细胞第1-7天的吸光度、S期、G2/M期和PI均显著低于CON组、DDP组和LEN组细胞(均P<0.05),G0/G1期和细胞凋亡率均显著高于CON组、DDP组和LEN组细胞(均P<0.05);LEN组和D+L组细胞MDR1和MRP1基因mRNA表达无明显差异(均P>0.05),CON组和DDP组细胞MDR1和MRP1基因mRNA表达显著高于LEN组和D+L组细胞(均P<0.05)。结论:香菇多糖体外可逆转卵巢癌细胞对顺铂耐药性,可能与降低MDR1和MRP1表达有关。

Objective：To study the reversing effect of lentinan on the resistance of A2780 ovarian cancer cellto Cisplatin in vitro and its mechanism.Methods：A2780ovarian cancer cells were divided into CON group（no Lentinan and Cisplatin treatment）,DDP group（only Cisplatin treatment）,LEN group（only lentinan treatment）,and D＋L group（both lentinan and Cisplatin treatment）according to their treatments.The differences of cell proliferation,cell cycle,apoptosis and expression of multi-drug resistance gene were compared.Results：The D1-D7 absorption,S stage,G2/M stage and PI in D＋L group were significantly lower than those in CON group,DDP group and LEN group（P〈0.05）,while G0/G1 stage and cell apoptosis rate were higher（P〈0.05）.The differences of MDR1 and MRP1’s mRNA expression were not statistics significant between LEN group and D＋L group（P〉0.05）,while their expression were lower than those in CON group and DDP group（P〈0.05）.Conclusion：Lentinan could reverse the resistance of A2780 ovarian cancer cell to Cisplatin in vitro via lowering down MDR1 and MRP1expression.