非小细胞肺癌中TRIM29、caspase-8、β-catenin蛋白表达情况及临床意义研究

Expression and clinical significance of TRIM29,caspase-8 and P-catenin in nonsmall cell lung cancer

目的探讨TRIM29、caspase-8和β-catenin在非小细胞肺癌(NSCLC)组织中的表达及其临床意义。方法分别通过免疫组织化学法检测254例NSCLC患者肺癌组织(NSCLC组)及其癌旁正常肺组织(对照组)中TRIM29、caspase-8和β-catenin表达情况,并分析其与患者临床特征的关系。结果 NSCLC组中TRIM29的阳性表达率和β-catenin的异常表达率均明显高于对照组,caspase-8的阳性表达率明显低于对照组(P﹤0.01);肺鳞癌组织中TRIM29阳性表达率高于肺腺癌组织,β-catenin的异常表达率低于肺腺癌组织,而caspase-8在两种组织中的表达无明显差异;TRIM29、caspase-8阳性表达患者的平均生存时间明显低于阴性表达者,β-catenin高表达患者的平均生存时间明显低于低表达患者(P﹤0.01);Cox多因素分析显示,淋巴结转移、肿瘤分期、TRIM29、caspase-8和β-catenin表达水平是影响患者生存的独立预后因素。结论 TRIM29、caspase-8、β-catenin在NSCLC中均有异常表达,同时与NSCLC患者淋巴结转移、病理分类、临床分期及患者生存期有密切关系,可以作为临床中判断NSCLC患者病理分类、分期及预后的参考依据。

Objective To explore the expression of TRIM29, caspase-8 andβ-catenin in non-small-cell lung cancer （NSCLC） and their clinical significance. Method Immunohistoehemical assay was used to detect the gene expression of TRIM29, caspase-8 and β-catenin in tumor tissues （NSCLC group） and adjacent normal tissues （control group） of 254 NSCLC patients, and the correlation with patient＆#39;s clinicopathological features was analyzed. Result The positive ex-pression rate of TRIM29 and abnormal expression rate ofβ-catenin in NSCLC tissues were significantly higher than that in control group, but the study group had lower expression rate of caspase-8 than control group （P〈0.01）;TRIM29 ex-pression in lung squamous carcinoma was higher than that in lung adenocarcinoma and the abnormal expression rate ofβ-catenin was lower in lung squamous carcinoma, while the caspase-8 expression in these two tissues was of no significant difference;Survival time of patients with positive TRIM29 and caspase-8 expressions was significantly lower than that of negative patients, and survival time of patients withβ-catenin expression was significantly lower than that of low-expres-sion patients （P〈0.01）. Cox multi-variate analysis showed that lymph node metastasis, tumor stage, TRIM29, caspase-8 and β-catenin expression levels were independent prognostic factors influencing the survival of patients. Conclusion The expression of TRIM29, caspase-8 and β-catenin were abnormal in NSCLC, and is closely correlated with NSCLC lymph node metastasis, pathological classification, clinical stage, as well as the patients＆#39;survival time, which can be used as references for pathological classification, clinical staging and prognosis of patients with NSCLC.