胰岛素联合肝细胞生长因子对肝切除术后肝再生的促进作用

Improve effect of insulin and hepatocyte growth factor on liver regeneration posthepatectomy

目的:观察经门静脉联合灌注胰岛素和肝细胞生长因子促进肝切除术后肝再生的效果。方法:将实验用新西兰兔随机分为胰岛素(INS)治疗组、肝细胞生长因子(HGF)治疗组、联合用药组(INS+HGF)、对照组,分别经门静脉置管微泵胰岛素、肝细胞生长因子、胰岛素和肝细胞生长因子、生理盐水,治疗结束时测定肝脏体积,计算肝脏再生速度。术后监测肝功能、血清TNF-α、IL-6。结果:联合治疗组较对照组及胰岛素组、肝细胞生长因子组肝功能恢复快(P<0.01),肝细胞生长因子组和胰岛素组较对照组肝功能恢复快(P<0.05)。术后残肝再生速度胰岛素组和肝细胞生长因子组均较对照组快(P<0.05),联合用药组较对照组和单一用药组显著增快(P<0.01)。实验组TNF-α、IL-6均较对照组升高,联合用药组与单一用药组比较有统计学意义。结论:经门静脉联合灌注胰岛素和肝细胞生长因子能显著促进肝切除术后肝再生。

Objective： To investigate the effect of insulin and hepatocyte growth factor on liver regeneration posthepatectomy by portal infusion. Methods： The animals undergone partial hepatectomy were assigned into INS group, HGF group, INS＋HGF group, control group. Four groups were treated by insulin, hepatocyte growth factor, insulin ＆ hepatocyte growth factor, 0.9% sodium chloride through the tube in portal vein. Liver volume, speed of liver regeneration, liver function, serum TNF-α and IL-6 were measured postoperation. Results： Recover of liver function in INS＋HGF group was faster than that in INS group and HGF group （P〈 0.01）. Recover of liver function in INS group and HGF group was faster than that in control group（P〈 0.05）.The speed of liver regeneration in control group was slower than that in HGF group and INS group （P〈 0.05）.The speed of liver regeneration in HGF＋INS group was rapider than that in HGF group and INS group （P〈 0.01）. Serum concentration of TNF-α and IL-6 in experimental groups was higher than that in control group, and HGF＋INS group was higher than HGF group and INS group. Conclusion： Infusion of insulin and hepatocyte by portal vein can significantly improve the liver regeneration posthepatectomy.