大鼠早期缺血心肌中Basigin mRNA的表达

The expression of basigin m RNA in early ischemic myocardium of rats

目的检测Basigin mRNA在大鼠早期缺血心肌和非缺血区心肌中的表达差异及探讨其法医学意义。方法建立大鼠早期心肌缺血手术模型,分为EIM组、NIM组、假手术组以及空白对照组,采用real-time PCR方法检测大鼠心肌缺血后15min、30min、1h和2h Basigin mRNA的表达量。结果与NIM组、假手术组及空白组相比,EIM组BSG mRNA表达量在缺血15min时降低,而冠状动脉结扎1h后表达量降低到SO组的一半,此时具有统计学差异(P<0.05),结扎2h后表达量恢复至SO组水平。NIM组、SO组和空白对照组之间表达量无统计学差异(P>0.05)。结论心肌急性缺血2小时内BSG mRNA表达降低明显,之后升高,说明BSG参与早期缺血心肌自我保护病理生理过程,提示BSG可用于鉴定早期心肌缺血。

Objective To determine the expression differences of Basigin（BSG）mRNA between early ischemic myocardium（EIM） and non-ischemic myocardium（NIM） in rats and then to evaluate the possibility of BSG examination in ischemic myocardium accidents occurred in forensic medicine. Methods Real-time polymerase chain reaction （RT-PCR） technique was applied for detecting the expression of BSG mRNA in ElM and NIM of rats at 15min, 30min, lh and 2h post myocardial ischemia, and in sham operation（SO） group. Results Compared with NIM, SO and control groups, expression of BSG mRNA decreased after 15min when myocardium ischemia occurred; compared with SO, it was of 0.5 folds when lh（P〈0.5）, and rebounded to SO level when 2h. Conclusion BSG could be involved in protection and myocardial remodeling in early ischemic myocardium, and may serves as a biomarker of early ischemic myocardium.